骨髓增生异常综合征患者骨髓CD34＋细胞亚群及其表面造血因子受体的表达  

Differentiation and expression of membrane hemopoietic cytokine receptors on CD34＋ bone marrow cells in patients with myelodysplastic syndromes

作　　者：李丽娟[1] 付蓉[1] 王化泉[1] 岳兰竹[1] 刘惠[1] 王琚[1] 王红蕾[1] 阮二宝[1] 瞿文[1] 梁勇[1] 王国锦[1] 王晓明[1] 刘鸿[1] 宋嘉[1] 吴玉红[1] 邢莉民[1] 关晶[1] 邵宗鸿[1] 

机构地区：[1]天津医科大学总医院血液肿瘤科,300052

出　　处：《中华医学杂志》2011年第4期234-238,共5页National Medical Journal of China

基　　金：国家“十一五”科技支撑计划（2008BAl61802）;天津市科技支撑计划重大疾病防治专项基金（07ZCGYSF00600）

摘　　要：目的探讨骨髓增生异常综合征（MDS）患者骨髓CD34＋细胞亚群及其表面干细胞因子受体（SCF－R）、红细胞生成素受体（EpoR）、粒细胞集落刺激因子受体（G－CSFR）及血小板生成素受体（TpoR）的表达情况及其意义。方法采用流式细胞术检测2008年7月至2010年3月天津医科大学总医院新诊断的45例MDS患者（17例低危患者、28例高危患者）及30名对照组原代骨髓CD34＋CD38＋及CD34＋CD38＋细胞亚群及其表面SCF－R、EpoR、G．CSFR及Tp0R的表达率。结果高危组CD34＋细胞比例[0．53％（0．10％～1．68％）]明显高于对照组[0．13％（0．08％～0．32％），P〈0．01]，其他2组问比较差异无统计学意义。低危组和高危组CD34＋CD38＋细胞比例（86．3％±8．5％、82．6％±11．1％）显著低于对照组（92．3％±3．4％，均P〈0．05），而CD34＋CD38＋细胞比例（13．7％±8．5％、17．4％±11．0％）显著高于对照组（7．7％±3．4％，均P〈0．05）。对照组骨髓CD34＋CD38＋细胞亚群EpoR表达率（18．7％±18．3％）显著低于CD34＋CD38＋细胞亚群（63．6％±20．O％，P〈0．01），两亚群之间SCF－R、G－CSFR及TpoR表达率差异无统计学意义。在CD34＋CD38＋细胞亚群中，3组间SCF－R和TpoR表达率差异无统计学意义，而低危组和高危组EpoR的表达率[9．0％（1．4％～12．7％）、5．2％（1．1％～14．1％）]明显低于对照组[9．6％（5．1％～30．1％），均P〈0．05]，G—CSFR的表达率（29．8％4-19．1％、28．7％±21．1％）明显低于对照组（44．4％4-23．4％，均P〈0．05）；在CD34＋CD38＋细胞亚群中，3组间SCF－R和G－CSFR表达率差异无统计学意义，低危组和高危组EpoR的表达率（42．2％±21．9％、25．7％±15．6％）明显低于对照组（63．6％±20．O％，均P〈0．01），TpoR的表达率（5．4％±4．7％、4．1％±4．0％）明显低于对照组（10．Objective To detect the abnormalities of differentiation and expression of membrane hemopoietic cytokine receptors on CD34＋ bone marrow cells in patients with myelodysplastic syndromes （MDS）. Methods Forty-five newly diagnosed MDS cases from July 2008 to March 2010 in our hospitaland 30 normal controls were enrolled. There were 17 low-risk and 28 high-risk patients. The CD34 ＋ CD38 ＋ and CD34 ＋ CD38- bone marrow cells and the expressions of stem cell factor receptor （SCF-R）, erythropoietin receptor （ EpoR ）, granuloeyte colony-stimulating factor receptors （ G-CSFR ） and thrnmbopoietin receptor （TpoR） on those cells were measured by flow eytnmetry. Results The mean percentage of CD34＋ in karyocyte of MDS eases in high-risk patients [0. 53% （0. 10% - 1.68% ） ] was significantly higher than that of control group [ 0. 13% （0. 08% - 0. 32% ）, P 〈 0. 01 ]. The mean percentages of CD34 ＋ CD38 ＋ cells were significantly lower in low and high-risk groups （86. 3% ± 8.5% and 82. 6% ± 11. 1% ） than those in control group （92. 3% ± 3.4% ）. And the percentage of CD34 ＋ CD38- cells was significantly higher in either low-risk or high-risk group （ 13.7% -＋ 8. 5% and 17.4% -＋ 11.0% ） than that in control group （7.7% ± 3.4% , both P 〈 0. 05 ）. In control group, the mean percentage of antigen expression of EpoR was significantly lower in CD34＋ CD38 ＋ cells than that in CD34＋ CD38＋ cells （ 18.7%± 18.3% vs 63.6% ± 20. 0% , P 〈 0. 01 ）. The expressions of SCF-R, G-CSFR and TpoR were not significantly different between two cell populations. The expressions of EpoR on CD34 ＋ CD38 ＋ cells of low and high-risk MDS groups [9. 0% （ 1.4% - 12. 7% ）, 5.2% （ 1.1% - 14. 1% ） ] were significantly lower than those of control group [9. 6% （5.1% -30. 1% ）, both P 〈0. 05]. The expressions of G-CSFR on CD34＋CD38＋ cells of low and high-risk MDS groups （29. 8% ± 19. 1% , 28. 7%± 21. 1%） were significantly lower than those of control group

关 键 词：骨髓增生异常综合征 抗原 CD34 原癌基因蛋白质C-KIT 受体 红细胞生成 素 受体 粒细胞集落刺激因子 

分 类 号：R551.3[医药卫生—血液循环系统疾病]