乙酰紫草素对培养大鼠心肌细胞的毒性作用  

Cytotoxicity effects of shikonin on rat cardiomyocytes in vitro

作　　者：桂春[1] 陈蒙华[1] 林松[1] 韦永先[1] 

机构地区：[1]广西医科大学第一附属医院西院心内科,南宁530021

出　　处：《中药药理与临床》2010年第6期33-36,共4页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：目的:检验乙酰紫草素(紫草素)对培养的大鼠心肌细胞的毒性作用以及明确其毒性作用的浓度。方法:取新生的SD大鼠心室的心肌细胞进行培养,然后用不同浓度的紫草素处理心肌细胞不同时间后,用MTT、台盼蓝拒染法、TUNEL和Hoechst染色检测其对心肌细胞的毒性作用,用W estern B lot检测凋亡蛋白Bc l-2和Bax的表达和Caspase3的激活。结果:当紫草素处理浓度小于1.3μM时,未发现明显的毒性作用,但当大于1.3μM时,毒性作用显现,当浓度在1.3到3μM之间时,随着浓度的逐渐增加,毒性作用也明显增加。用1.9μM和2.5μM的浓度的紫草素处理心肌细胞12小时后,Bc l-2表达明显减少,Bax表达明显增多,剪切的Caspase3明显增多。结论:大于1.3μM浓度的紫草素对培养的大鼠心肌细胞有毒性作用,其毒性作用也有凋亡机制的参与。Objective： To test the toxicity effects and toxicity concentrations of shikonin on cultured rat cardiomyocytes.Methods： The neonatal rat ventricular myocytes were isolated and cultured,and treated with various concentrations of shikonin for the indicated time periods.then the drug toxicity of shikonin on cardiomyocytes was measured by MTT assay,trypan blue uptake assay,TUNEL and Hoechst staining.Furthermore,Western Blot was made to examine the changes of apoptosis passage protein,such as Bcl-2/Bax ratio and caspase3 activation.Results： There were no significant toxicity effects on cardiomyocytes when the concentration of shikonin was less than 1.3μM.but more than 1.3μM,shikonin appeared toxicity.The toxicity effects were dose dependent,from 1.3μM to 3μM,the higher were shikonin concentrations,the severer were toxicity effects.Cardiomyocytes were treated with 1.9μM and 2.5μM shikonin for 12 hours,the Bcl-2 expression of Cardiomyocytes was decreased significantly,and Bax expression of Cardiomyocytes was increased significantly,and Cleaved-caspase3 also was increased significantly.Conclusion： There are significant toxicity effects on cardiomyocytes when the concentrations of shikonin are more than 1.3μM,and the toxicity effects are dose dependent.Apoptosis mechanism contributes to the toxicity effects.

关 键 词：紫草素 心肌细胞 毒性作用 凋亡 

分 类 号：R285[医药卫生—中药学]