Differential expression of genes encoding sulfur metabolism-related periplasmic proteins of Acidithiobacillus ferrooxidans ATCC 23270  

作　　者：夏金兰 张瑞永 张倩 武顺 张成桂 聂珍媛 邱冠周 

机构地区：[1]Key Laboratory of Biometallurgy of Ministry of Education of China,School of Minerals Processing and Bioengineering,Central South University

出　　处：《Transactions of Nonferrous Metals Society of China》2010年第12期2366-2370,共5页中国有色金属学报（英文版）

基　　金：Projects(50674101,50974140)supported by the National Natural Science Foundation of China;Project(2010CB630902)supported by the National Basic Research Program of China

摘　　要：Reverse-transcription qualitative PCR(RT-qPCR)was used to analyze the changes in transcription levels of the sulfur metabolism-related periplasmic protein genes of Acidithiobacillus ferrooxidans ATCC 23270 grown on sulfur or ferrous.Seven periplasmic proteins with apparently higher abundance grown on elemental sulfur than on ferrous sulfate were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF-MS).Expression analysis of the corresponding genes by RT-qPCR shows that the constitutive expression of all those genes are more up-regulated grown on sulfur than those grown on ferrous(>10 folder).Study on the corresponding genes of the identified periplasmic proteins by RT-qPCR confirmed the results of two-dimensioned gel electrophoresis,indicating they may be related with sulfur metabolism in A.ferrooxidans.Reverse-transcription qualitative PCR（RT-qPCR）was used to analyze the changes in transcription levels of the sulfur metabolism-related periplasmic protein genes of Acidithiobacillus ferrooxidans ATCC 23270 grown on sulfur or ferrous.Seven periplasmic proteins with apparently higher abundance grown on elemental sulfur than on ferrous sulfate were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry（MALDI-TOF-MS）.Expression analysis of the corresponding genes by RT-qPCR shows that the constitutive expression of all those genes are more up-regulated grown on sulfur than those grown on ferrous（10 folder）.Study on the corresponding genes of the identified periplasmic proteins by RT-qPCR confirmed the results of two-dimensioned gel electrophoresis,indicating they may be related with sulfur metabolism in A.ferrooxidans.

关 键 词：Acidithiobacillus ferrooxidans RT-QPCR periplasmic proteins 

分 类 号：TF18[冶金工程—冶金物理化学]