小鼠巨噬细胞吞噬马尔尼菲青霉酵母相细胞的实验观察  

Phagocytosis of Penicillium marneffei in yeast phase by macrophages of mice

作　　者：冯佩英[1,2] 黄怀球[2] 张静[1,2] 张晓辉[2] 孙九峰[1] 谢治[1] 鲁莎[1] 鲁长明[1] 席丽艳[1] 

机构地区：[1]中山大学附属第二医院皮肤科,广州510120 [2]中山大学附属第三医院皮肤科,广州510630

出　　处：《中国真菌学杂志》2010年第6期332-335,共4页Chinese Journal of Mycology

基　　金：国家自然科学基金(30770121)

摘　　要：目的探讨小鼠腹腔巨噬细胞对马尔尼菲青霉酵母相细胞的影响,为研究抗马尔尼菲青霉的免疫机制提供一定的实验依据。方法将马尔尼菲青霉酵母相细胞与小鼠腹腔巨噬细胞在体内和体外两种方式下进行共培养,分别于60min、120min、240min和360min后进行菌落形成单位(CFU)计数,统计分析体内组与体外组CFU数结果的差异性。利用钙荧光白染色巨噬细胞胞内的马尔尼菲青霉酵母相细胞,荧光显微镜下观察细胞形态特征的变化。结果经体内和体外两种方式共培养,两组之间的CFU计数差异无统计学意义,但两组内不同时间点的CFU计数差异存在统计学意义。荧光显微镜下可见巨噬细胞内的酵母细胞被钙荧光白染上淡蓝色荧光。结论小鼠腹腔巨噬细胞在体内、外对马尔尼菲青霉酵母相细胞不起明显的杀灭或溶解作用,其最大的吞噬时间为共培养240min。钙荧光白染色可快速有效的区分巨噬细胞与真菌,是一种良好的检测真菌方法。Obejective To study the effect of peritoneal macrophages of BALB/c mice on phagocytosis of Penicillium marneffei in yeast phase.Methods Yeast-phase P.marneffei and peritoneal macrophages of BALB/c mice were cultured both in vitro and in vivo.The number of colony forming unit（CFU） was counted at 60 min,120 min,240 min and 360 min respectively.The difference of the CFU numbers between the in vitro and in vivo groups was then analyzed.Calcofluor white stain was applied to observe the morphology of yeast cells.Results CFU numbers in four time points in each group differed significantly（P〈0.05）,while the difference of CFU numbers between the in vitro and in vivo groups showed no significance（P〉0.05）.P.marneffei in yeast phase showed light blue by calcofluor white stain under fluorescence microscope.Conclusions No obvious destroy or lysis exists in P.marneffei when co-cultured with macrophages.Calcofluor white stain is preferred for identification of macrophage and yeast cells.

关 键 词：巨噬细胞 马尔尼菲青霉 菌落形成单位 钙荧光白染色 

分 类 号：R379.9[医药卫生—病原生物学]