妊娠相关血清蛋白-A对内皮细胞生理功能的影响  

Effect of pregnancy-associated plasma protein-A on the function of endothelial cells

作　　者：彭丽萍[1] 王建刚[1] 杨侃[1] 蒋卫红[1] 严文广[2] 易斌[3] 

机构地区：[1]中南大学湘雅三医院心内科,长沙410013 [2]中南大学湘雅三医院康复科,长沙410013 [3]中南大学湘雅三医院肾内科,长沙410013

出　　处：《中南大学学报（医学版）》2010年第12期1261-1265,共5页Journal of Central South University ：Medical Science

摘　　要：目的:观察妊娠相关血清蛋白-A(PAPP-A)在体外对内皮细胞生理功能的影响。方法:体外培养人脐静脉内皮细胞株(HUVEC),随机分为4组。分别给予不同浓度(0,50,100及200ng/mL)的PAPP-A刺激,于0,12,24和48h收集上清液和细胞,用硝酸还原酶法测定一氧化氮(NO)的浓度,用免疫组织化学法测定内皮素-1(ET-1)的表达。结果:在不同的时间点,随着PAPP-A浓度的增加,上清液NO的浓度逐渐降低,与对照组相比差异具有统计学意义(P<0.05)。随着PAPP-A浓度的增加,ET-1的表达呈浓度依赖性增加,与对照组相比差异具有统计学意义(P<0.05)。结论:PAPP-A可能通过减少内皮细胞NO的分泌,上调ET-1的表达来影响内皮细胞的功能。Objective To determine the effect of pregnancy-associated plasma protein A （PAPP-A） on the function of vascular endothelial cells （VEC）. Methods Human umbilical vein endothelial cell （HUVEC） line, derived from human umbilical vein, was cultured in vitro with PAPP-A at 0, 50, 100, and 200 ng/mL for 0, 12, 24, and 48 hours, respectively. Nitric oxide （NO） levels and endothlin-1 （ET-1） levels were determined by spectrophotometer and immunehistory. Results The NO levels of HUVECs in the PAPP-A groups were significantly lower than those in the control group （P0.05）. The ET-1 levels of HUVECs in the PAPP-A groups were significantly higher than those in the control group （P0.05）. The changes were all dose-dependent. Conclusion PAPP-A may affect the function of vascular endothelial cells by reducing the secretion of NO and increasing the level of ET-1.

关 键 词：妊娠相关血清蛋白-A 内皮细胞 一氧化氮 内皮素-1 

分 类 号：R543.5[医药卫生—心血管疾病]