地塞米松对水通道蛋白1、4、5在大鼠变应性鼻炎鼻黏膜中表达的影响及意义  

Effect of dexamethasone on aquaporin 1, 4, 5 expression in rat nasal mucosa of allergic rhinitis

作　　者：许海波[1] 郑鸣[2] 李兆生[1] 

机构地区：[1]福建医科大学附属漳州市医院耳鼻喉科,漳州363000 [2]福建医科大学人体解剖学与组织胚胎学系,福州350004

出　　处：《解剖学杂志》2010年第6期729-731,743,共4页Chinese Journal of Anatomy

基　　金：福建医科大学科学研究发展基金

摘　　要：目的：确定水通道蛋白（AQPs）在大鼠变应性鼻炎时鼻黏膜上的表达及分布,并观察地塞米松对AQPs表达的影响,探讨AQPs与变应性鼻炎时水液分泌的关系.方法：健康SD大鼠随机分成空白组、假处理组、模型组、干预组,组织化学方法观察各组组织形态学特征,免疫组织化学方法（SP法）检测各组AQPs的表达并进行比较.结果：AQP1在血管内皮细胞、成纤维细胞中表达较强;AQP4表达于上皮细胞和上皮内腺上皮细胞及部分上皮下的腺上皮细胞;AQP5在上皮下部分腺上皮细胞成簇表达.经地塞米松处理后,AQP1、4、5的表达均有明显变化,仅AQP4在干预组间的表达并无明显改变.结论：AQP1、4、5在大鼠鼻黏膜上有不重叠表达;地塞米松能调节AQPs在鼻黏膜上的表达.Objective： To determine the expression of aquaporins （AQPs） in nasal mucosa of allergic rhinitis （AR）, to observe the effect of dexamethasone （DEX） on the expression of AQPs in the allergic rhinitis and to explore the relation of AQPs and the fluid transportation in rats. Methods： Healthy adult rats were randomly divided into a blank group, a control group, a model group, and a hormone-intervention group. With histochemistry staining, we observed the histomorphologic character of AR. The expression changes of AQPs in the nasal mucosa in each group were investigated with immunohisto-chemical technique （SP method）. Results： AQP1 expressed intensively in capillary and blood sinus endothelial cells, and the fibroblasts. AQP4 was localized at the basaolateral membrane in the epithelial cells, endoepithelial gland and the part of the subepithelial gland. AQP5 resided in apical membrane of the part of subepithelial obviously. After the treatment of DEX, the expression of AQP1, 4, 5 changed obviously, compared to the model group. The expression of AQP4 in the 1-week DEX group had no significant difference versus 2-week DEX group. Conclusion ： The protein of AQP1, 4, 5 presented at different sites. DEX may regulate the expression of AQP1, 4, 5 in the nasal mucosa.

关 键 词：水通道蛋白 变应性鼻炎 地塞米松 免疫组织化学 大鼠 

分 类 号：R563[医药卫生—呼吸系统]