当归对宫内缺氧新生大鼠神经元的影响及机制  

Effect of angelica on neuron of neonatal rats after intrauterin hypoxia and its mechanism

作　　者：陈旭东[1] 华新宇[1] 陈世丰[1] 余鸿[2] 

机构地区：[1]漯河医学高等专科学校组织学与胚胎学教研室,漯河462002 [2]泸州医学院组织学与胚胎学教研室,泸州646000

出　　处：《解剖学杂志》2010年第6期771-773,共3页Chinese Journal of Anatomy

基　　金：四川省卫生厅课题（060052）

摘　　要：目的：探讨宫内缺氧对新生大鼠神经元分化的影响及当归的调控作用.方法：将孕SD大鼠随机分为对照组、缺氧组和当归治疗组.于孕14d开始将当归组与缺氧组孕鼠置于低张氧浓度三气培养箱中,制作胎鼠宫内缺氧模型.3组孕鼠分娩当日取脑组织行神经元烯醇化酶（NSE）和血管内皮生长因子（VEGF）原位杂交,DAB显色.结果：缺氧组新生鼠NSE阳性细胞表达较对照组减少,而VEGF mRNA的表达较对照组增加;当归组NSE mRNA和VEGF mRNA阳性细胞表达较缺氧组增加.结论：当归注射液对宫内缺氧新生大鼠脑组织具有保护作用,其作用机制可能是通过上调VEGF mRNA的表达而改善缺氧环境所致.Objective： To study the effect and its mechanism of angelica on neuron of neonatal rat after intrauterin hypoxia. Methods： Female SD rats were divided randomly into three groups： control group, hypoxia group and angelica group. On the 14th day of rat pregnant, the rats in the hypoxia and angelica groups were put into the incubator to establish a model of intrauterine anoxia. Then the brain tissues of neonatal rats were immediately taken out after childbirth. The expressions of neuron-specific enolase （NSE） and vascular endothelial growth factor （VEGF） were detected by in situ hybridization. Results： The integrated optical density （IOD） of NSE mRNA positive cells in the hypoxia group was obviously less than that in the control group; IOD of VEGF mRNA positive cells was obviously higher than that in the control group; IOD of NSE and VEGF mRNA positive ceils in the angelican group was higher obviously than that in the hypoxia group. Conclusion： Angelica injection has protective effect on neuron of neonatal rat, which may possibly upregulate the expression of VEGF mRNA to ameliorate the intrauterin hypoxic environment.

关 键 词：当归 缺氧 脑 血管内皮生长因子 新生大鼠 

分 类 号：R285.5[医药卫生—中药学]