大蒜油对环磷酰胺化疗小鼠白细胞减少症的预防作用  

Preventive effect of garlic oil on cyclophosphamide-induced leukopenia in tumor-bearing mice

作　　者：李阳[1] 闫捷[1] 付强强[1] 赵盛[1] 张翠丽[1] 于丽华[1] 谢克勤[1] 

机构地区：[1]山东大学公共卫生学院卫生毒理学研究所,济南250012

出　　处：《山东大学学报（医学版）》2010年第12期153-157,共5页Journal of Shandong University:Health Sciences

基　　金：山东省科技攻关计划项目(2008GG2NS02012)

摘　　要：目的探讨大蒜油(GO)对环磷酰胺(CP)化疗荷瘤小鼠白细胞减少症的预防作用。方法 60只昆明小鼠随机分为荷瘤模型组(Model组)、CP组、25μg/gGO+CP组(GO1+CP组)和50μg/gGO+CP组(GO2+CP组),共4组。右侧腋窝下接种H22瘤细胞,连续给药13d。观测体质量、瘤重、脾重、脾结节计数、外周血象、骨髓DNA含量、骨髓有核细胞计数及骨髓嗜多染红细胞微核率变化。结果与Model组相比,CP组瘤重减少60.5%(P<0.05),白细胞计数降低65.9%(P<0.05),脾脏指数、脾结节计数、骨髓DNA含量、骨髓有核细胞计数分别降低了34.8%、63.1%、50.1%、64.3%(P<0.05),骨髓微核率升高到40.2‰(P<0.05)。GO1+CP组和GO2+CP组抑瘤率分别为58.1%、62.0%,与CP组差异无统计学意义(P>0.05)。与CP组比较,GO2+CP组白细胞计数显著增加(P<0.05),脾脏指数、脾结节计数、骨髓DNA含量、骨髓有核细胞计数分别升高了60.0%、161.3%、39.7%、140%(P<0.05),其骨髓微核率降低至28.6‰(P<0.05)。结论大蒜油在保证环磷酰胺抑瘤作用下,可明显减轻白细胞减少症等化疗不良反应。Objective To observe the preventive effect of Garlic oil（GO）on H22 tumor-bearing mice in conjunction with cyclophosphamide（CP）chemotherapy.Methods 60 mice were randomly divided into four groups：the tumor model,the CP group,the 25 μg/g GO ＋CP and the 50 μg/g GO＋CP group.Each mouse was subcutaneously inoculated with Hepatoma 22 cells in the right armpit,followed by 13 days of GO administration.Mice were sacrificed by cervical dislocation,and then tumor mass and spleens were dissected and weighed.In addition,WBC,spleen index,spleen nodes count,the contents of DNA and Nucleated marrow cells（NMC）in bone marrow,bone marrow micronucleus were detected.Results Compared with the tumor model,the tumor mass of the CP group decreased by 60.5%,while WBC,spleen index,spleen nodes count,the contents of DNA in bone marrow and NMC were lowered by 65.9%,34.8%,63.1%,50.1%,64.3%（P〈0.05）,respectively.Compared with the CP group,50 μg/g GO caused a tumor-inhibiting rate of 62.0%（P〉0.05）,and increased WBC,spleen index,spleen nodes count,the content of DNA and NMC（P〈0.05）.Conclusions Garlic oil can attenuate leukopenia induced by CP on tumor-bearing mice without influencing its anti-tumor activity.

关 键 词：大蒜油 环磷酰胺 H22 化疗 小鼠 

分 类 号：R979.1[医药卫生—药品] R-332[医药卫生—药学]