血清高尔基体糖蛋白73在肝细胞癌诊断中的作用  

Detection and evaluation of serum GP73, a resident Golgi glycoprotein, as a marker in diagnosis of hepatocellular carcinoma

作　　者：赵秀英[1] 李宁[1] 丁惠国[1] 姜菲菲[1] 

机构地区：[1]首都医科大学附属北京佑安医院临床检验中心,100069

出　　处：《中华肿瘤杂志》2010年第12期943-946,共4页Chinese Journal of Oncology

基　　金：国家自然科学基金（30973388）

摘　　要：目的 探讨血清高尔基体糖蛋白73（GP73）在肝细胞癌（HCC）早期诊断中的作用.方法 采用酶联免疫吸附试验（ELISA）定量检测43例体检正常者（正常对照）、110例慢性肝炎或肝硬化（CH/LC）患者和219例HCC患者血清中GP73水平,计算ROC曲线下面积及GP73对HCC诊断的敏感性与特异性.结果 正常对照组、CH/LC组和HCC组GP73分别为（22.1±8.5）ng/ml、（81.4±57.2）ng/ml和（271.5±202.3）ng/ml,HCC组GP73水平明显高于CH/LC组（P＜0.001）.GP73的ROC曲线下面积是0.857,以100 ng/ml作为cut-off值,GP73诊断HCC的敏感性为76.7%,特异性为73.2%.在149例甲胎蛋白（AFP）＜400 ng/ml的HCC样本中,有108例（72.5%）GP73＞100 ng/ml.对4例AFP＜400ng/ml的HCC患者连续检测表明,GP73与治疗效果及预后有一定的相关性.结论 GP73可作为HCC早期诊断及治疗效果监测的指标,尤其对AFP＜400 ng/ml的HCC患者有较好临床意义.Objective The aim of this study was to determine if Golgi protein-73（GP73）is upregulated in hepatocellular carcinoma（HCC）, and to explore the possibility of using GP73 in diagnosis and treatment of HCC. Methods Serum GP73 was detected by a quantitative ELISA assay. A total of 372 serum samples were included, among them 43 from healthy donors（Normal）, 110 from either chronic hepatitis or cirrhosis（CH/LC）, and 219 from HCC patients. The levels of GP73 were compared among the 3 groups. The received operating curve（ROC）, sensitivity and specificity of GP73 for HCC patients were calculated. Results The average level of GP73 expression in normal, CH/LC and HCC groups were（22.1 ±8.5）ng/ml,（81.4 ± 57.2）ng/ml and（271.5 ± 202.3）ng/ml, respectively. Serum GP73 levels were significantly higher in patients with HCC compared to those with CH/LC（P 〈0.001）. The GP73 area under ROC was 0. 857. Put 100 ng/ml as the optimal cut-off point, GP73 had a sensitivity of 76.7% and a specifically of 73.2%. GP73 level had a significantly higher sensitivity than AFP（32.0%）in diagnosis of early HCC（P 〈 0. 001）. Moreover, GP73 level was elevated in the serum（72. 5% , 108/149）of individuals with HCC who had serum AFP level less than 400 ng/ml. Following-up study of 4 HCC patients with low level AFP indicated that GP73 was associated with treatment and prognosis of HCC. Conclusion Higher level of GP73 can be found in the serum of patients with HCC than those without. GP73 is better than AFP for the diagnosis of early HCC and in evaluating treatment result in patients with normal AFP. Further studies may help to validate both the role and mechanism of GP73 in diagnosis of HCC.

关 键 词：癌 肝细胞 诊断 高尔基体糖蛋白73 甲胎蛋白 

分 类 号：R686[医药卫生—骨科学]