淋巴液引流对创伤失血性休克大鼠多器官损伤的影响  

Effects of lymph drainage on multiple organ injury of rats with traumatic hemorrhagic shock

作　　者：牛春雨[1] 赵自刚[1] 张玉平[1] 杜舒婷[1] 常海峰[1] 张扬[1] 闫斌[1] 尚金星[1] 陈锦霞[1] 

机构地区：[1]河北北方学院微循环研究所/基础医学院病理生理学教研室,张家口075029

出　　处：《中华创伤杂志》2010年第12期1144-1148,共5页Chinese Journal of Trauma

基　　金：国家自然科学基金资助项目（30370561;30770845）;河北省自然科学基金资助项目（C2004000649;C2008000503）;河北北方学院重大科研项目（ZD200901）

摘　　要：目的 观察肠淋巴液引流对创伤失血性休克（T/HS）大鼠多器官损伤的影响,探讨其作用机制.方法 Wistar雄性大鼠分为对照组、淋巴液引流组与淋巴液回流组,后两组复制T/HS模型,淋巴液引流组行肠淋巴液引流.观察各组大鼠平均动脉血压（MAP）,检测反映酸碱状态以及肝、肾、心肌的生化指标,对肺、肝、肾、心肌进行病理观察并检测其ATP含量和ATPase活性的变化.结果 淋巴液引流组在输液80 min后多个时相点的MAP、多个组织器官的ATP含量、ATPase活性均高于淋巴液回流组,淋巴液引流组大鼠的多项生化指标优于淋巴液回流组,差异有统计学意义;淋巴液回流组可见炎症、淤血、变性、坏死等变化,而淋巴液引流组病变轻微.结论 休克淋巴液引流可减轻T/HS大鼠的多器官损伤,其机制与改善能量代谢、维持血压和酸碱状态有关.Objective To observe the effect of shock lymph drainage on multiple organ injury of rats with traumatic hemorrhagic shock （THS） and discuss the relating mechanism. Methods Male Wistar rats were divided into control group, lymph drainage group and lymph return group. The THS model was established in lymph drainage group and lymph return group, when the shock mesenteric lymph was drained in lymph drainage group. The change of the mean arterial pressure （ MAP）, the biochemical indices of liver, kidney, myocardium and acid-base, the morphology, ATP contents and ATPase activities of lung, kidney, liver and myocardium were observed. Results The MAP at multiple time points after 80 minutes of infusion, the ATP contents and ATPase activities of multiple organs in lymph drainage group were higher than those in lymph return group. Multiple biochemical indices in lymph drainage group were superior to those in lymph return group, with statistical difference. The inflammation, congestion, degeneration and necrosis were found in organs of lymph return group, but only mild lesions could be seen in lymph drainage group. Conclusions The shock lymph drainage can alleviate multiple organ injury of THS rats, mechanism of which is correlated with improvement of the energy metabolism and maintenance of MAP and acid-base status.

关 键 词：休克 出血性 肠系膜淋巴液 引流术 能量代谢 大鼠 Wistar 

分 类 号：R686[医药卫生—骨科学]