海绵共栖细菌抗菌活性及其群体感应信号的研究  

The study on antibacterial activity of sponge-associated bacteria and quorum-sensing signal molecules

作　　者：郭秀春[1,2] 郑立[2] 崔志松[2] 杨佰娟[2] 田黎[2] 王小如[1,2] 

机构地区：[1]中国海洋大学化学化工学院,山东青岛266003 [2]国家海洋局第一海洋研究所海洋生态研究中心,山东青岛266061

出　　处：《海洋学报》2010年第5期135-143,共9页

基　　金：国家自然科学基金(20602009;40776098);海洋"863"项目(2007AA09Z435);国家海洋局海洋公益项目(200805039);青岛市科技发展技术(08-1-3-10-JCH);中国科学院海洋研究所海洋生态与环境重点实验室开放课题的资助

摘　　要：利用平板涂布法从南海海绵生物样品中分离海绵共栖细菌,以枯草芽孢杆菌、金黄色葡萄球菌、大肠杆菌及酿酒酵母作为实验用指示菌对海绵共栖细菌进行了抗菌活性筛选。利用气相质谱联用仪(GC-MS)建立了群体感应信号分子酰基高丝氨酸内酯类化合物(AHLs)的检测方法,分析了活性菌株粗提液中信号分子的产生及种类。同时采用16S rDNA同源性和系统发育分析对活性菌株进行种属鉴定和系统发生学研究。结果表明,在分离到的94株海绵共栖细菌中18株(占19.1%)细菌具有抗菌活性。通过GC-MS检测确定大多数活性菌株都含有AHLs,因此提出了海绵共栖细菌抗菌活性与群体感应之间可能存在必然联系的推测。通过细菌分类鉴定,结果显示具有抗菌活性的细菌大部分属于芽孢杆菌属(Bacillus)。Sponge-associated bacteria were isolated from marine organism sponge by dilution-plate method,and the antibacterial activities were screened for Bacillus subtilis,Staphylococcus aureus,Escherichia coli and Saccharomyces cerevisia by an agar diffusion method.And the occurrence of quorum-sensing signal molecule acylhomoserine lactones（AHLs） was investigated in the extracts of active strains by a gas chromatography-mass spectrometry.At the same time,taxonomy was studied based on the phylogenetic analysis of 16S rDNA sequences.The results show that 18 strains（accounting for 19.1%） among total 94 isolated marine bacteria have antimicrobial activity.The N-octanoyl-homoserine lactone and N-tetradecanoyl-homoserine lactone were detected from the majority of active strains,showing that the some relationship between the antimicrobial activity and the quorum sensing.The most active sponge-associated bacteria were identified to be the genus Bacillus.

关 键 词：海绵共栖细菌 抗菌活性 群体感应 酰基高丝氨酸内酯 分子鉴定 

分 类 号：Q935[生物学—微生物学] O657.7[理学—分析化学]