新型甲型H1N1流感病毒HA基因DNA疫苗诱导小鼠产生中和抗体  

DNA vaccines encoding hemagglutinin of novel H1N1 influenza A virus inducing neutralization antibody in mice

作　　者：邵圣文[1] 周洪昌[1] 徐伯赢[1] 刘小青[2] 方静[2] 王岳[3] 陈志辉[4] 

机构地区：[1]湖州师范学院医学院微生物学教研室,313000 [2]第二军医大学微生物学教研室 [3]中国疾病预防控制中心病毒病预防控制所、分子病毒与遗传工程国家重点实验室 [4]第二军医大学长海医院感染科,上海200433

出　　处：《中华微生物学和免疫学杂志》2010年第12期1114-1117,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家863高技术研究发展计划（2007AA02Z157）

摘　　要：目的 探讨新型甲型流感病毒（2009H1N1）血凝素（HA）DNA疫苗诱导小鼠产生中和抗体特性.方法 构建2009H1N1或1918甲型流感病毒（1918H1N1）HA蛋白表达质粒2009HA和1918HA,采用25μg或200μg剂量2009HA质粒免疫小鼠,以2009HA或1918HA蛋白为包被抗原,测定小鼠血清中2009HA抗体总量或交叉反应抗体含量,分别用2009H1N1和1918H1N1两种假病毒（pp）测定抗体中和活性.结果 25 μg或200μg的2009HA质粒加强免疫小鼠后,4～16周内两组小鼠血清中2009HA总抗体水平以及对2009H1N1pp的中和抗体滴度相似（P〉0.05）,都含有与1918HA蛋白交叉反应抗体,对1918H1N1pp的交叉中和抗体滴度相似（P〉0.05）.结论 小剂量2009HA质粒DNA疫苗能够诱导小鼠产生持久的高水平中和抗体,对于预防新现流感病毒具有潜在应用价值.Objective To study the characteristics of neutralization antibody in mice induced by DNA vaccines of hemagglutinin（HA） of novel H1N1 influenza A virus（2009H1N1）.Methods HA encoding plasmids of 2009H1N1 or 1918H1N1（2009HA or 1918HA）were constructed.25 μg or 200 μg dosage of 2009HA plasmids were used to immunize the mice,the total antibody of anti-20O9HA or cross-reactive antibody were assayed by ELISA using 2009HA or 1918HA protein as capture antigen,and the neutralizing antibody were assayed by two kinds of virus pseudo - particles（pp） of 2009H1N1 and 1918H1N1 .Results During of 4 to 16 weeks after boost immunization,in two groups of mice immunized with 25 μg or 200 μg dosage 2009HA plasmids,both total antibody of anti-2009HA and neutralizing antibody to 2009H1Nlpp reached the similar level（P 〉0.05）,and there were cross-reactive antibody to 1918HA protein in two groups of mice serum,with similar titers of cross-neutralizing activity to 1918H1N1 pp（P 〉0.05）,Conclusion A low dosage DNA vaccine encoding HA of 2009 H1 N1 virus is able to induce persistent and high level of neutralizing antibody,and may be potential valuable vaccine against the new emerging influenza virus.

关 键 词：新型甲型流感病毒 血凝素 DNA疫苗 中和抗体 假病毒 

分 类 号：R3[医药卫生—基础医学]