检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
机构地区:[1]上海交通大学医学院附属国际和平妇幼保健院,上海200030
出 处:《生殖与避孕》2010年第12期812-817,共6页Reproduction and Contraception
摘 要:目的:了解半乳糖凝集素-3(galectin-3)在早孕期绒毛与蜕膜中的表达,为进一步探讨稽留流产发病机制提供理论依据。方法:分别获取手术流产组(n=20)、药物流产组(n=20)及稽留流产组(n=20)患者的流产绒毛及蜕膜,用免疫组织化学法及实时荧光相对定量PCR法检测各组绒毛及蜕膜组织中半乳糖凝集素-3的表达。结果:免疫组织化学显示稽留流产组绒毛半乳糖凝集素-3强阳性表达率(30.00%)明显低于手术流产组(80.00%)(P<0.01)。RT-PCR结果进一步证实稽留流产组绒毛半乳糖凝集素-3 mRNA的相对表达量(2-△△Ct=0.31±0.70)明显低于手术流产组(2-△△Ct=1.00±0.00)(P<0.01),药物流产组蜕膜半乳糖凝集素-3 mRNA的相对表达量(2-△△Ct=0.74±2.20)明显低于手术流产组(2-△△Ct=1.00±0.00)(P<0.01)。结论:半乳糖凝集素-3与妊娠早期绒毛及蜕膜发育有关,其表达降低可能促进了稽留流产的发生。Objective:To detect the expression of galectin-3 in villus and decidua of early pregnancy,and explore the pathogenesis of missed abortion.Methods:Sixty cases of villus and decidua were divided into 3 groups:surgical abortion group(n=20),medical abortion group(n=20) and missed abortion group(n=20).The expression of galectin-3 in villus and decidua of each group was measured by methods of immunohistochemis-try and real-time fluorescence relative quantitative PCR.Results:Immunohistochemistry showed that galectin-3 strongly-positive expression in villus of missed abortion group(30.00%) was significantly lower than that of surgical abortion group(80.00%)(P0.01).Real-time fluorescence relative quantitative PCR showed that galectin-3 mRNA relative expression in villus of missed abortion group(2-△△Ct =0.31±0.70) was significantly lower than that of surgical abortion group(2-△△Ct =1.00±0.00).Galectin-3 mRNA relative expression in decidua of medical abortion group(2-△△Ct =0.74 ± 2.20) was significantly lower than that of surgical abortion group(2-△△Ct =1.00±0.00)(P0.01).Conclusion:Galectin-3 is related to the development of villus and decidua during early pregnancy.The decreased expression of galectin-3 may promote the occurrence of missed abortion.
关 键 词:半乳糖凝集素-3(galectin-3) 早期胚胎发育 绒毛 蜕膜 稽留流产
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.200