氯化锂联合脐血干细胞移植修复大鼠脊髓损伤  

Lithium chloride combined with human umbilical cord blood mesenchymal stem cell transplantation for treatment of spinal cord injury in rats

作　　者：邓许勇[1] 周荣平[1] 鲁凯伍[2] 金大地[1] 

机构地区：[1]南方医科大学第三附属医院脊柱外科,广东广州510630 [2]南方医科大学南方医院脊柱骨病外科,广东广州510515

出　　处：《南方医科大学学报》2010年第11期2436-2439,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30471761);广东省自然科学基金(04020415)~~

摘　　要：目的观察氯化锂联合脐血干细胞移植对大鼠脊髓全横断损伤的修复效果。方法成年雌性SD大鼠80只,建立胸9脊髓全横断损伤模型,随机分对照组(A组,n=20)、氯化锂组(B组,n=20)、细胞移植组(C组,n=20)和氯化锂+细胞移植组(D组,n=20)。于术后1d、3d及每周的最后1天,应用BBB评分评价大鼠脊髓功能的恢复情况;8周后取材,通过Brdu细胞核标记观察移植细胞的存活、迁移;通过荧光金逆行追踪,观察脊髓神经纤维的再生与分布。结果 4只大鼠死亡。术后8周可观察到Brdu标记的脐血干细胞在体内存活并在脊髓内迁移;细胞移植组和氯化锂+细胞移植组可见少量连续性神经纤维通过损伤区。荧光金逆行脊髓追踪显示C组和D组可见被荧光金标记的神经锥体细胞穿越损伤区。术后8周A、B、C、D四组后肢功能运动BBB评分分别为4.11±0.14、4.50±0.15、8.31±0.11、11.15±0.18。结论氯化锂能促进人脐血间充质干细胞在损伤区的存活并向神经细胞分化,氯化锂联合脐血干细胞与脊髓去细胞支架移植能够促进细胞移植修复大鼠脊髓损伤的效果。Objective To observe the effects of lithium chloride combined with human umbilical cord blood mesenchymal stem cell（hUCB-SCs） transplantation in the treatment of spinal cord injury in rats.Methods Eighty female SD rats with complete T9 spinal cord transaction were randomized into 4 groups（n=20）,namely the control group（group A）,lithium chloride group（group B）,hUCB-SCs group（group C） and hUCB-SCs＋ lithium chloride group（group D）.On days 1 and 3 and the last days of the following weeks postoperatively,the motor function of the hindlimb of the rats were evaluated according to the BBB scores.At 8 weeks,all the rats were sacrificed and the spinal cords were taken for morphological observation.The spinal cord tissues at the injury site were observed with Brdu nuclear labeling to identify the survival and migration of the transplanted SCs.The regeneration and distribution of the spinal nerve fibers were observed with fluorescent-gold（FG） spinal cord retrograde tracing.Results Brdu labeling showed that the transplanted hUCB-SCs survived and migrated in the spinal cord 8 weeks postoperatively in groups C and D.FG retrograde tracing identified a small amount of pyramidal cells that migrated across the injury site in groups C and D.The BBB scores of the hindlimb motor function 8 weeks postoperatively were 4.11±0.14,4.50±0.15,8.31±0.11 and 11.15±0.18 in groups A,B,C and D,respectively.Conclusion Lithium chloride can promote the survival and differentiation of hUCB-SCs into neural cells at the injury site.Lithium chloride combined with hUCB-SCs transplantation may accelerate functional recovery of the hindlimbs in rats with complete transection of the spinal cord.

关 键 词：脊髓损伤 氯化锂 脐血 干细胞移植 

分 类 号：R651.2[医药卫生—外科学]