三黄茵赤汤对急性肝衰竭大鼠肝脏损害及凋亡效应酶caspase3的影响  

Effect of Sanhuangyinchi decoction on liver damage and caspase-3 in rats with acute hepatic failure

作　　者：杨运高[1] 刘亚伟[1] 华何与[1] 李莉洁 

机构地区：[1]南方医科大学中医药学院,广东广州510515 [2]解放军第425中心医院,海南三亚572008

出　　处：《南方医科大学学报》2010年第11期2443-2445,共3页Journal of Southern Medical University

基　　金：国家自然科学基金(30070937)~~

摘　　要：目的研究三黄茵赤汤对急性肝衰竭大鼠肝脏损害及凋亡效应酶caspase3的影响。方法用氨基半乳糖,内毒素脂多糖复制大鼠急性肝衰竭模型,随机分为正常对照组、模型组、安宫牛黄组和三黄茵赤组,分别观察病死率、丙氨酸氨基转移酶、血清总胆红素、凝血酶原时间和caspase3。结果模型组36h病死率74.29%(26/35),安宫牛黄组和三黄茵赤组为31.43%(11/35)和28.57%(10/35),与模型组比较差异均有统计学意义(均P<0.01),安宫牛黄组和三黄茵赤组可以显著降低ALT、TBIL、PT、caspase3水平(P<0.01),在降低TBIL、caspase3的作用上三黄茵赤汤优于安宫牛黄丸组(P<0.05)。结论三黄茵赤汤能显著改善肝功能,降低caspase3活性,这可能是其临床治疗重症肝炎有效的机制之一。Objective To investigate effect of Sanhuangyinchi decoction（SHYCD） on liver damage and the pro-apoptotic factor caspase-3 in rats with acute hepatic failure.Methods SD rats were random1y divided into blank control group,model group,Angongniuhuang group（AGNH） and SHYCD group.Acute hepatic failure was induced in the rats by intraperitoneal injections of D-GaLN and LPS,and the death rate,ALT,TBIL,PT and caspase-3 activity was observed or tested.Results At 36 h after the injections,the death rate of the rats was 74.29%（26/35） in the model group,31.43%（11/35） in AGNH group and 28.57%（10/35） in SHYCD group.The death rate,ALT,TBIL,PT and caspase-3 activity in AGNH and SHYCD groups were significantly lower than those in the model group（P〈0.01）.SHYCD showed stronger effect than AGNH in depressing TBIL and the activity of caspase-3（P〈0.05）.Conclusion SHYCD can improve the liver function and inhibit the activity of caspase-3 in rats,which can be the possible mechanism for its therapeutic effect against acute hepatic failure.

关 键 词：急性肝衰竭 CASPASE3 三黄茵赤汤 

分 类 号：R285.5[医药卫生—中药学]