人类白细胞抗原-G在急性白血病患者的表达及意义  

Clinical implications of HLA-G protein expression in acute leukemia

作　　者：李蕊[1] 曹星辉[1] 刘灿[1] 吴秀丽[1] 凌奕文[1] 张钰[1] 冯茹[1] 刘启发[1] 

机构地区：[1]南方医科大学南方医院血液科,广东广州510515

出　　处：《南方医科大学学报》2010年第11期2446-2448,共3页Journal of Southern Medical University

基　　金：国家自然科学基金(30971300);广东省社会发展基金(2009A030200007);广州市重大科技专项计划(2008A1-E4011-1)~~

摘　　要：目的检测急性白血病患者膜结合型人类白细胞抗原-G(mHLA-G)和分泌型HLA-G(sHLA-G)的表达,探讨HLA-G表达水平的变化对急性白血病发生和发展的影响。方法采用酶联免疫吸附法和流式细胞术分别检测40例初发、10例难治复发、30例化疗前后患者及10例正常人sHLA-G和mHLA-G的表达情况。结果正常人血清中sHLA-G的平均水平为5.87±2.07ng/ml,初发患者为10.05±6.5ng/ml、难治复发患者为12.32±5.85ng/ml;正常人mHLA-G的平均水平为(0.29±0.20)%,初发患者(0.60±0.44)%、难治复发患者(0.77±0.41)%。白血病初发患者sHLA-G和mHLA-G水平均显著高于正常人组(P<0.05),初发与难治复发患者比较无统计学差异(P>0.05)。15例1疗程化疗后获得完全缓解患者sHLA-G和mHLA-G水平显著低于化疗前(P<0.05),而15例未缓解患者其sHLA-G和mHLA-G水平较治疗前无明显差异(P>0.05)。结论 HLA-G表达水平变化可能与急性白血病的病情发展、疗效以及预后有关。Objective To detect the expression of membrane-bound HLA-G（mHLA-G） and serum HLA-G（sHLA-G） in acute leukemia patients and investigate the correlation between HLA-G expression and the occurrence and development of acute leukemia.Methods Enzyme-linked immunosorbent assay and flow cytometry were used to detect the expression levels of sHLA-G and mHLA-G in 40 newly diagnosed leukemia cases,10 refractory and relapsed leukemia cases,and 30 leukemia cases receiving chemotherapy.Ten normal individuals served as the normal control group.Results The mean serum level of sHLA-G in normal individuals was 5.87±2.07 ng/ml,as compared to 10.05 ± 6.58 ng/ml in newly diagnosed leukemia patients and 12.32±5.85 ng/ml in refractory and relapsed cases.The mean level of mHLA-G in normal individuals,newly diagnosed cases,and refractory and relapsed cases were（0.29±0.20）%,（0.60±0.44）%,and（0.77±0.41）%,respectively.The mean levels of sHLA-G and mHLA-G were significantly higher in the newly diagnosed cases than that in the normal controls（P〈0.05）,and significantly higher in patients before chemotherapy than in those with complete remission after chemotherapy（P〈0.05）.Conclusion HLA-G expression levels might influence the treatment outcomes and can serve as a prognostic factor for acute leukemia.

关 键 词：白血病 急性 人类白细胞抗原-G 预后 

分 类 号：R392[医药卫生—免疫学] R733.7[医药卫生—基础医学]