分层复合骨-软骨支架的制备及生物相容性初步研究  

Preparation and biocompatibility evaluation of osteochondral composite scaffold

作　　者：王秋玲[1] 谢德明[1] 吕玉明[2] 

机构地区：[1]暨南大学生物医学工程系,广东广州510632 [2]广州医学院第三附属医院骨科,广东广州510150

出　　处：《南方医科大学学报》2010年第11期2477-2481,共5页Journal of Southern Medical University

基　　金：广东省自然科学基金(7002690);广东省医学科研基金(A2007277);广州医学院科研资助(0709106)

摘　　要：目的制备分层复合骨-软骨支架及研究其生物相容性。方法首先制备纳米羟基磷灰石(nano-HAP)/I型胶原复合材料,以nano-HAP/I型胶原/聚乳酸-羟基乙酸共聚物(PLGA)为骨部分支架,透明质酸钠/PLGA为软骨部分支架,分层复合构建骨-软骨支架,透射电镜、红外光谱、X射线衍射分析nano-HAP/I型胶原复合材料颗粒粒径大小、化学组成及结晶度,扫描电镜观察支架微观形貌,并通过大鼠骨髓基质干细胞-支架复合培养、噻唑蓝法检测支架的生物相容性及细胞毒性。结论分层复合骨-软骨支架具有良好的微观结构,无细胞毒性,细胞与支架生物相容性良好,适合作为骨-软骨支架。Objective To prepare osteochondral composite scaffold and study its biocompatibility in vitro.Methods The composite material of nano-HAP/collagen I was prepared,and the osteochondral scaffold was manufactured by combining nano-HAP,collagen I,and PLGA as the bone section and sodium hyaluronate and PLGA as the chondral section.The diameter,chemical composition and crystallinity of the nano-HAP/collagen I composite particles were assessed with TEM,FTIR and XRD,and the biocompatibility and cytoxicity of the scaffold were evaluated using MTT assay by co-culturing bone marrow stem cells and the scaffold.Results and Conclusion The osteochondral composite scaffold has good microstructure without obvious cytoxicity,possesses good biocompatibility with bone marrow stem cells and is suitable as an osteochondral scaffold material.

关 键 词：纳米羟基磷灰石 骨髓基质干细胞 噻唑蓝 生物相容性 

分 类 号：R318.08[医药卫生—生物医学工程]