全麻复合硬膜外阻滞对高血压大鼠心肌c-fos和HSP70基因表达的影响  

Effect of epidural block combined with general anesthesia on c-fos and HSP70 expression in the myocardium of spontaneous hypertensive rats

作　　者：李真[1] 王庆[1] 王志鹏[1] 崔灿[1] 

机构地区：[1]广东省人民医院/广东省医学科学院麻醉科,广东广州510080

出　　处：《南方医科大学学报》2010年第11期2492-2494,共3页Journal of Southern Medical University

基　　金：广东省医学科研基金(A2009025)

摘　　要：目的通过检测高血压大鼠心肌组织c-fos和HSP70基因的表达水平,研究全麻复合硬膜外阻滞减轻应激反应的效果。方法 18只SHR和18只SD大鼠随机分3组:气管切开全麻复合硬膜外阻滞组(A1、A2)、气管切开全麻组(B1、B2)和对照组(D1、D2)。全麻以氟芬合剂(0.1～0.3ml/kg)复合地西泮(3～6mg/kg)腹腔注射,气管切开连接简易呼吸装置。硬膜外阻滞选择T11-T12棘突间隙切开直接向头端插入硬膜外导管2cm,缓慢注入1%利多卡因0.2ml。A1、A2、B1、B2组施行脾脏切除术。尾动脉置管连接监护仪监测MAP、HR,同时观察呼吸改变。术后24h处死动物,取心肌组织2块,液氮保存。用RT-PCR方法检测c-fos和HSP70两基因反应产物相对于β-actin值。结果高血压大鼠全麻复合硬膜外阻滞组(A1)的c-fos/β-actin比值显著低于单纯全麻组(B1),而HSP70/β-actin比值显著高于全麻组。结论与单纯全麻比较,全麻复合胸段硬膜外阻滞可以减少高血压大鼠手术刺激时心肌c-fos表达、降低应激反应水平,同时增强HSP70表达,对心肌具有一定的保护作用。Objective To study the efficacy of epidural block combined with general anesthesia on stress reaction in spontaneous hypertensive rats（SHR）.Methods Eighteen SHR and 18 SD rats（above 24 weeks） were randomly divided into 6 groups,namely epidural block combined with general anesthesia group（A1,A2）,general anesthesia group（B1,B2） and control group（D1,D2）.General anesthesia was performed with celiac injection of droperidol,fentanyl and diazepam.Tracheal intubation and ventilation were performed after tracheotomy,and epidural block was conducted by incision.The rats in groups A1,A2,B1,B2 underwent splenectomy.All the rats were sacrificed 24 h after the surgery and two myocardium specimens were collected for detecting c-fos and HSP70 expression using RT-PCR.Results c-fos mRNA expression was significantly lower but HSP70 mRNA expression significantly higher in group A1 than in group B1.Conclusion Compared with general anesthesia,general anesthesia combined with epidural block can reduce the stress responses and protect cardiac myocytes by decreasing c-fos expression and increasing HSP70 expression in SHR.

关 键 词：硬膜外麻醉 高血压 C-FOS 热休克蛋白类 

分 类 号：R614.2[医药卫生—麻醉学]