压应力对组织工程软骨细胞增殖的影响  

Proliferation of tissue-engineered cartilage cells under compressive stress

作　　者：李殿威[1] 周强[1] 郭平[1] 宋磊[1] 刘涌[1] 

机构地区：[1]第三军医大学西南医院骨科,重庆400038

出　　处：《南方医科大学学报》2010年第11期2530-2532,2538,共4页Journal of Southern Medical University

摘　　要：目的探讨不同强度的压应力对组织工程软骨细胞增殖的影响。方法将兔软骨细胞接种到脱钙骨基质支架上,应用仿生多功能组织工程组织培养仪,加压组分别在压缩幅度为0～5%、0～10%、0～20%,频率0.1Hz周期性压力下培养,对照组不加压。通过大体观察、组织切片、MTT法及流式细胞术分别观察组织形态、细胞数量与分布、检测细胞增殖活力及细胞周期。结果 0～10%组的组织工程软骨体积最大、最厚,形态佳,组织切片见软骨细胞数目最多,分布更为均匀,排列紧密,基质分泌较多。加压组的软骨细胞活力显著增强,S期细胞百分比均明显高于对照组,以0～10%组尤为明显。结论组织工程软骨细胞增殖能力受周期性压力强度的调控,其中频率0.1Hz、强度0～10%的周期性压力能更好地促进软骨细胞增殖。Objective To investigate the proliferation of tissue-engineered cartilage cells stimulated by different intensities of compressive stress.Methods Human embryonic cartilage cells were seeded into type II collagen sponge scaffold.The cells of the pressure groups were stimulated by different compression rate（0-5%,0-10%,and 0-20%） at a cyclical frequency of 0.1 Hz.The cells of the control group were cultured without pressure.Gross observation,histological section,MTT assay and flow cytometry were used to observe the morphology,cell number and distribution and detect the cell proliferation and cell cycle.Results Tissue-engineered cartilage cells in 0-10% group showed the largest size and greatest thickness with normal morphology.Tissue biopsies showed the largest number of cartilage cells with more uniform distribution,close alignment,more matrix secretion.The cartilage cell activity was significantly enhanced and the percentage of S phase cells significantly increased in the pressure group compared with those in the control group,and such changes were especially obvious in 0-10% group in which the S phase cells increased by 59.0% compared with that in the control group.Conclusion The proliferation of tissue-engineered cartilage cells is regulated by the cyclic stress intensity,and a pressure frequency of 0.1 Hz with compression rate of 0-10% can better promote the cell proliferation.

关 键 词：压应力 软骨细胞 细胞增殖 组织工程 

分 类 号：R318.01[医药卫生—生物医学工程] R322.71[医药卫生—基础医学]