科罗索酸提取工艺初步研究  

An optimized method for extracting corosolic acid from loquat leaves

作　　者：贺建荣[1] 刘欠[2] 白志龙[2] 黄仁权[1] 王增禄[1] 

机构地区：[1]第四军医大学唐都医院药剂科,陕西西安710038 [2]第四军医大学药学系生物技术中心,陕西西安710032

出　　处：《南方医科大学学报》2010年第11期2533-2535,共3页Journal of Southern Medical University

摘　　要：目的建立从枇杷叶中提取具有药用价值的科罗索酸提取方法。方法以枇把叶为原料,水煮去除水溶性物质,25%甲醇除杂,95%甲醇在80℃回流提取1h,重复提取3次,合并提取液,冷却到室温,活性炭脱色,过滤,回收甲醇,浓缩产生沉淀,水洗沉淀,静置使固液分离,沉淀物即为总三萜酸粗品,以甲醇溶解粗品,过滤,色谱分离,获得科罗索酸馏分,减压回收溶剂,干燥后即可获得科罗索酸产品。用高效液相色谱法检测分析科罗索酸。结果经HPLC分析,醇提粗品中科罗索酸为4.66%,水提物中科罗索酸为2.42%,低醇提取物中几乎全为杂质,去杂后的醇提物中科罗索酸为16.98%,经HPLC纯化后的科罗索酸纯度大于80%。结论经对提取方法的优化组合,建立了从枇杷叶中提取具有药用价值的科罗索酸提取方法,获得纯度达到80%的科罗索酸。该制备方法简单、生产成本低,具有推广价值。Objective To extract corosolic acid from loquat leaves for medical use.Methods Loquat leaves were boiled in water to remove the water-soluble substances followed by 3 cycles of extraction with 25% aqueous methanol for 30 min and then by 95% aqueous methanol for 1 h at 80 ℃.After cooling at room temperature and filtration,the extract was treated with activated carbon to remove chlorophyll,and the liquid was filtered and concentrated to allow precipitation.The sediment was washed to obtain the total crude triterpene acid,which was further dissolved with methanol and purified by high-performance liquid chromatography（HPLC）.The fractions including korosolic acid were collected,concentrated with vacuum distillation,and dried to obtain corosolic acid product,which was analyzed with HPLC.Results HPLC analysis of the extracts showed that the percentages of corosolic acid were 4.66%,2.42%,and 24.18% in crude corosolic acid extracted with methanol,boiling water,and 95% aqueous methanol,respectively.After purification with HPLC,the purity of corosolic acid in the product exceeded over 80%.Conclusion The optimal extraction method,which is convenient and cost-effective,is established for extracting corosolic acid from loquat leaves for medical use.

关 键 词：枇杷叶 科罗索酸 HPLC 

分 类 号：R284.2[医药卫生—中药学]