HPLC法测定五指毛桃中补骨脂素、佛手柑内酯、芹菜素的含量  

High-performance liquid chromatography for determination of psoralene,bergapten and apigenin in Ficus hirta Vahl

作　　者：杨燕军[1] 代军 

机构地区：[1]广东食品药品职业学院,广东广州510520 [2]浙江华海药业公司,浙江临海317024

出　　处：《南方医科大学学报》2010年第11期2565-2567,共3页Journal of Southern Medical University

基　　金：广东省科技计划项目(2006B035530008;2008B030301195);广东省自然科学基金(9151063201000036)

摘　　要：目的建立同时测定五指毛桃中补骨脂素、佛手柑内酯、芹菜素含量的高效液相色谱方法。方法应用HypersilC18色谱柱(250mm×4.6mm,5μm),流动相为甲醇-水(60:40),流速为1.0ml/min,检测波长为268.7nm,柱温为30℃。结果补骨脂素的线性范围为10.0～30.0μg/ml(r=0.9998),样品的加样回收率为99.7%,RSD1.99%;佛手柑内酯的线性范围为15.0～45.0μg/ml(r=0.9998),样品的加样回收率为99.9%,RSD1.71%;芹菜素的线性范围为5.0～15.0μg/ml(r=0.9992),样品的加样回收率为100.3%,RSD1.78%。结论本方法简便快速,回收率、重复性良好,可用于五指毛桃中补骨脂素、佛手柑内酯、芹菜素的含量分析。Objective To establish a high-performance liquid chromatography（HPLC）-based method for determining the contents of psoralene,bergapten and apigenin in Ficus hirta Vahl.Methods A Hypersil C18 column（250 mm×4.6 mm,5 μm） was used with the mobile phase of methanol-water（60：40）,flow rate of 1.0 ml/min,detection wavelength of 268.7 nm and column temperature of 30 ℃.Results The calibration curve was linear within the range of 10.0-30.0 μg/ml for psoralene（r= 0.9998）,15.0-45.0 μg/ml for bergapten（r=0.9998） and 5.0-15.0 μg/ml for apigenin（r=0.9992）.The average recovery of psoralene was 99.7%（RSD=1.99%）,that of bergapten was 99.9%（RSD=1.71%） and that of apigenin was 100.3%（RSD= 1.78%）.Conclusion The method is simple,economic and accurate with good reproducibility for the contents of psoralene,bergapten and apigenin.

关 键 词：五指毛桃 补骨脂素 佛手柑内酯 芹菜素 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]