严重烫伤内毒素血症SD大鼠胰岛β细胞功能检测与GIK干预研究  

Studies on the Determination of Islets β Cell in Scalded Rats with LPS-injected and the Effects of GIK Treatment

作　　者：王占科[1,2] 常律[1] 汪仕良[3] 许霖水[3] 刘龙燕[2] 胡扬涛[2] 余文[2] 

机构地区：[1]天津大学,天津300072 [2]解放军第九四医院,南昌330002 [3]卫生部中日友好医院内分泌科,北京100029

出　　处：《实验动物与比较医学》2010年第6期420-423,共4页Laboratory Animal and Comparative Medicine

基　　金：国家科技支撑课题（2008BAI52B03）;江西省科技支撑项目（2008BAO7400）;南京军区医学科技技术＂十一五＂计划重点项目（06Z25）;江西省卫生科技计划项目（20052044）

摘　　要：目的 研究严重烫伤内毒素血症大鼠胰岛β细胞功能不全及GIK（极化液）的干预效果.方法 SD大鼠90只,制作30%体表面积（TBsA）Ⅲ°烫伤内毒素血症大鼠,随机分为烫伤内毒素血症组（n=30）,极化液干预组（n=30）,对照组（n=30）.测定烫伤内毒素血症组伤前和伤后不同时相点以及极化液干预组伤后7 d空腹血胰岛素、血糖、腹腔给糖30min后胰岛素和血糖水平,计算并观察稳态模式评估法胰岛素分泌指数（HOMA-β）、糖负荷后胰岛素增量和血糖增量比值（△ INS30/△ GLU30）以及胰岛素抵抗指数（HOMA-IR）的变化.结果 烫伤内毒素血症组伤后1、3、5和7 d空腹血糖、空腹胰岛素均明显高于伤前,HOMA-β指数和△INS30/△ GLU30比值均明显低于伤前,HOMA-IR指数明显高于伤前（均P〈0.01）.极化液干预组空腹血糖明显低于其治疗对照组,HOMA-β指教和△INS30/△GLU30比值均明显高于其治疗对照组,HOMA-IR指数无显著性差别.结论 严重创伤内毒素血症机体胰岛β细胞基础胰岛素分泌和糖负荷后早期胰岛素分泌功能均明显下降,早期使用极化液/胰岛素对胰岛β细胞功能有保护作用.Objective To study the dysfunction of islets β cell in scalded rats with LPS-injected and the effects of GIK（glucose-insulin- Potassium） treatment to the islets β cell. Methods Ninety SD scalded rats with LPS-injected were divided randomly into the S＋LPS（n=30）, GIK （n=30） and control groups（n=30）. The glucose and insulin of the fasting and 30rain post of glucose injected of the all groups were determined, and the HOMA-β, △ INS30/A GLU30 and HOMA-IR were calculated and observed. Results The glucose and insulin of the fasting and 30min post of glucose injected and the HOMA-IR of the S＋LPS group were higher than that of the before scalded（P〈0.01）, but the HOMA-β and △ INS30/ △ GLU30 of the S＋LPS group were lower than that of the before scalded（P〈0.01）. The HOMA-β and △ INS30/ △ GLU30 of the GIK group were higher than that of the control group, there was no difference of HOMA-IR significantly between the GIK and control group. Conclusion The serious bum rats represents dysfunction of early phase and basal insulin release of β cell, GIK treatment would be beneficial for the protect of β cell on this states.

关 键 词：烧伤 胰岛β细胞功能不全 极化液 效果 

分 类 号：R644[医药卫生—外科学] R-332[医药卫生—临床医学]