基于“酶-邻氨基苯酚”体系的电化学免疫传感方法及其应用  

An Electrochemical Immunosensor Based on "Enzyme O-aminophenol" System and Its Application

作　　者：胡海燕[1] 邓王平[1] 宋世平[1] 樊春海[1] 

机构地区：[1]中国科学院上海应用物理研究所,上海201800

出　　处：《生物物理学报》2010年第12期1130-1136,共7页Acta Biophysica Sinica

基　　金：卫生部国家重大科技专项(2009ZX10004-301;2009ZX10004-302)~~

摘　　要：本研究建立了一种新的电化学免疫传感方法,并将其应用于实际样本的检测。采用辣根过氧化物酶(horseradish peroxidase,HRP)的新型底物邻-氨基苯酚(O-aminophenol,O-AP)构建了基于"酶-邻氨基苯酚"体系的电化学免疫传感方法,并用于血吸虫抗体检测。邻-氨基苯酚在辣根过氧化物酶催化双氧水(H2O2)时被氧化,生成的产物3-氨基吩噁嗪能通过电化学方法检测。实验结果验证了基于"酶-邻氨基苯酚"的新型蛋白检测体系用于检测人血清中血吸虫抗体的可操作性,实现了实际样本的检测。并且,采用方波伏安法作为最终检测方法,具有更高的灵敏性。This study develops a new electrochemical immunosensor,using O-aminophenol（O-AP）that is a new substrate of horseradish peroxidase（HRP）,which is called＂Enzyme O-aminophenol＂system.The immunosensor was used for the detection of Schistosoma japonicum antibodies.O-AP is oxidized with the catalytic action of HRP.The oxidative product and electrode reduction reaction form the voltammetry enzyme-linked immune analysis system.After the enzyme catalysis,the 3-aminophenoxazine product could be detected by electrochemistry method.The results indicated that the new protein detection method based on＂Enzyme O-aminophenol＂is feasible for the detection of Schistosoma japonicum antibody in human serum with higher sensitivity.

关 键 词：电化学免疫传感 血吸虫抗体 酶联免疫分析 

分 类 号：Q554.6[生物学—生物化学]