腺相关病毒介导血管抑素对血管内皮生长因子和细胞间黏附分子1表达的影响  

The effect of rAAV-AS on expression of VEGF and ICAM-1 in HUVECs

作　　者：徐维[1] 李伟[2] 

机构地区：[1]徐州医学院,221002 [2]徐州医学院附属医院

出　　处：《中华老年心脑血管病杂志》2011年第4期361-364,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：江苏省高校自然科学基金(06KJB320117)

摘　　要：目的探讨腺相关病毒介导血管抑素（rAAV_AS）基因对人脐静脉内皮细胞（HUVECs）血管内皮生长因子（VEGF）和细胞间黏附分子1（1CAM-1）表达的影响，以探讨rAAV—AS抗糖尿病动脉粥样硬化作用。方法选择体外培养的HUVECs传至第5代，随机分为6组：正常组、高糖组、高糖＋rAAV-010。v．g．／cell组、高糖＋rAAV—AS10^4v．g．／cell组、高糖＋rAAVAS10^5v．g．／cell组、高糖＋rAAV—AS10^6v．g．／cell组。分别检测rAAV—AS干预后24、48、72hHUVECs凋亡及VEGF、ICAM-1的表达。结果与正常组比较，高糖组和高糖＋rAAV010^8v．g．／cell组不同时间HUVECs增殖明显，VEGF和ICAM-1表达明显升高（P〈0．05）；与高糖组比较，高糠＋rAAV-AS10^4v.g./cell组、高糠＋rAAV-AS10%5v.g.cell组、高糠＋rAAV-AS^10^5v.g./cell组增殖明显降低，VEGF和ICAM-1表达明显降低，并呈剂量依赖性（P〈0．05）。结论rAAV—AS对糖尿病大血管病变具有保护作用，其可能机制为下调内皮细胞中VEGF和ICAM-1的表达，从而减少内皮细胞增殖，抑制炎性反应。Objective To investigate effects of recombinant adeno associated virus carrying human angiostatin gene（rAAV-AS） on expression of vascular endothelial growth factor（VEGF） and inter-cellular adhesion moleculel （ICAM-1） in human umbilical vein endothelial cells（HUVECs）, and its mechanisms of anti-diabetic atherosclerosis. Methods HUVECs cells were cultured in vitro ,and the fifth passage cells were randomly divided into six groups：normal group, hyperglycemia group,hyperglycemia＋rAAV-010^6 v.g. group,hyperglycemia＋rAAV-AS10^4 v.g. group, hyperglycemiaq-rAAV-AS10^5 v.g. group,and hyperglycemia＋rAAV-AS10^6 v.g. group. The apoptosis of HUVECs and expression of VEGF and ICAM-1 were detected 24,48 and 72 h after rAAV-AS intervention. Results Significant inhibition of proliferation of HUVECs and expression of VEGF and ICAM-I in a dose-dependent manner was observed in 3 rAAV AS transfected groups compared with hyperglycemia group. Conclusion rAAV-AS has protective effect against pathological changes of large vessels in diabetes, its mechanism may be down-regulation of expression of VEGF and ICAM-1 in endothelial cells,thus reducing proliferation of endothelial cells and inhibiting inflammatory reaction.

关 键 词：腺病毒科 血管内皮生长因子类 细胞黏附分子 脐静脉 内皮细胞 糖尿病 动脉粥样硬化 

分 类 号：R587.2[医药卫生—内分泌] R543.5[医药卫生—内科学]