钼营养对甘草生理和生长特性的影响  

Effect of Different Concentrations of Mo on Characteristic of Physiology and Growth of Glycyrrhiza uralensis Fisch

作　　者：王丹[1] 万春阳[1] 王文全[1,2] 方玉强[1] 陈惠杰[1] 刘影[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]中药材规范化生产教育部工程研究中心,北京100102

出　　处：《现代生物医学进展》2011年第4期632-636,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金面上项目(30572328);北京中医药大学在读研究生自主选题项目

摘　　要：目的:探讨不同浓度的钼营养水平对甘草生长和生理特性的影响。方法:以一年生的甘草移栽苗为试验材料,采用盆栽蛭石的试验方法,共设置4个钼浓度水平,分别为0mg·L-1,0.52mg·L-1,5.2mg·L-1和10.4mg·L-1,其中0.52mg·L-1即正常Hoagland营养液中钼的浓度。每周向盆内浇灌营养液,以达到处理的目的。采用LI-6400光合仪测定其光合生理指标以及植物生理学常规方法进行甘草叶片色素和抗氧化酶活性的测定。采用电子天平分别测定不同处理下的甘草地上、根的鲜重和干重等。结果:结果表明,甘草的各项生理和生长指标随着钼处理浓度的增加而增加,显著增加了甘草植株的叶绿素a,总叶绿素,类胡萝卜素和净光合速率(Pn)、胞间CO2浓度(Ci)等光合指标以及显著提高超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性,统计分析结果表明,各处理间差异显著(P<0.05)。同样,钼营养显著增加了甘草株高、芦头直径、生物量等生长指标。其中,10.4mg·L-1钼处理时的甘草根鲜重和干重最大,与0 mg·L-1处理相比,分别显著增加了55.35%和38.08%。结论:钼不足会抑制甘草的各项生理功能,进而影响甘草的生长,而5.2mg·L-1和10.4mg·L-1的钼营养浓度可以促进一年生甘草各项生理和生长指标的增加,进而促进甘草干物质的积累,提高甘草药材的产量。Objective：To investigate the effects of different concentrations of molybdenum（Mo） on the characteristic of physiology and growth of Glycyrrhiza uralensis Fisch.Method：The transplants of one-year-old G.uralensis were subjected to four concentrations of Mo（0,0.52,5.2 and 10.4 mg·L-1 Mo） culturing in vermiculite and 0.52 mg·L-1 Mo was used as the normal concentration in the complete Hoagland nutrition solution.The solution was irrigated to the pots every week.The photosynthetic physiological indicators were mea-sured by LI-6400 photosynthetic instrument.The pigments and antioxidase activities of the leaves were determined by general method in plant physiology.The fresh and dry weights of shoots and roots of the plants were measured by electronic balance.Result：Several physi-ological and growth indicators increased with the increasing of Mo concentrations.The application of Mo significantly increased chloro-phyll a,total chlorophyll,cartenoid,and the photosynthetic indicators of Pn,Ci.And the superoxide dismutas（SOD）,peroxidase（POD）,catalase（CAT） increased significantly with the increasing of Mo.Meanwhile,Mo significantly increased the growth indicators of height,root diameter and biomass and so on.The fresh and dry weights of the roots under 10.4 mg·L-1 Mo treatment reached the highest com-pared with 0 mg·L-1 Mo treatment,they increased by 55.35% and 38.08%.Conclusions：The deficiency of Mo could inhibit the physio-logical function and so affected the growth of the plants.In this study,the concentration of 5.2 mg·L-1 and 10.4 mg·L-1 could promote the increase of physiological and growth indicators and enhance the accumulation of dry materials to increase the output of the Chinese medicine material.

关 键 词：钼 甘草 光合特性 抗氧化酶活性 生长 

分 类 号：S567.71[农业科学—中草药栽培] R931.2[农业科学—作物学]