己糖激酶介导小豆蔻明改善血管平滑肌细胞糖代谢的作用  

Improvement Effects of Cardamonin on Glucose Metabolism of Vascular Smooth Muscle Cells Induced by Hexokinase

作　　者：牛培广[1] 张钰璇[2] 史道华[2] 

机构地区：[1]福建医科大学福总临床医学院药学科,福建福州350108 [2]南京军区福州总医院药学科,福建福州350025

出　　处：《现代生物医学进展》2011年第4期653-656,共4页Progress in Modern Biomedicine

基　　金：福建省自然科学基金项目(2008J0103)

摘　　要：目的:探讨小豆蔻明对胰岛素抵抗状态(IR)血管平滑肌细胞(VSMCs)糖代谢的影响及其作用机制。方法:采用高糖(2.5×l0-2 mol·L-1)高胰岛素(100 U·L-1)培养VSMCs,72 h后加入小豆蔻明培养48 h,观察培养液中葡萄糖消耗量、细胞内己糖激酶活性以及细胞增殖。结果:高糖高胰岛素培养72h后,血管平滑肌细胞糖消耗量降低(P<0.01);小豆蔻明能显著性增加IR细胞的平均糖消耗量(P<0.01),增强己糖激酶活性(P<0.01);同时明显抑制高糖高胰岛素引起的VSMCs增殖(P<0.01)。结论:小豆蔻明能增强己糖激酶活性,改善IR状态下细胞糖代谢;同时抑制高糖高胰岛素刺激引起的VSMCs异常增殖。Objective：To investigate the effects of cardamonin on regulation of glucose metabolism on the vascular smooth mus-cle cells（VSMCs） and its possible mechanism in the insulin-resistant condition.Methods：VSMCs were cultured in high glucose（2.5×l0-2 mol·L-1） and high insulin（100 U·L-1） medium for 72 hours,then cardamonin was added to incubate for 48 hours.Glucose consump-tion,hexokinase activity and proliferation of VSMCs were measured in the experiment.Results：Cultured with high glucose and high in-sulin for 72 hours,glucose consumption of VSMCs was significantly decreased（P0.01）.Cardamonin significantly increased the glucose consumption and hexokinase activity of VSMCs in IR statue（P0.01） and inhibited the proliferation induced by high glucose and high insulin（P0.01）.Conclusion：The results suggested that cardamonin could improve the glucose metabolism and inhibit the proliferation of VSMCs in the IR condition,which might be related to enhancement of hexokinase activity.

关 键 词：小豆蔻明 胰岛素抵抗 糖消耗 己糖激酶 

分 类 号：R965[医药卫生—药理学] R977.15[医药卫生—药学]