妊娠期肝内胆汁淤积症患者胎盘组织中有机阴离子转运多肽-A,-C表达的研究  

Placental Expression of Organic Anion Transporting Polypeptide-A and-C in Intrahepatic Cholestasis of Pregnancy

作　　者：刘玉芳[1] 门晓亮[1] 崔陶[1] 吴琳[1] 邢爱耘[1] 

机构地区：[1]四川大学华西第二医院妇产科,四川成都610041

出　　处：《中华妇幼临床医学杂志（电子版）》2011年第2期86-89,共4页Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)

基　　金：国家自然科学基金资助项目(30571964)~~

摘　　要：目的探讨有机阴离子转运多肽(organic aniontransporting polypeptide,OATP)-A,-C在妊娠各期和妊娠期肝内胆汁淤积症(intrahepatic cholestasis of pregnancy,ICP)患者胎盘的表达。方法收集2006年5月至2007年2月,在本院产科确诊为肝内胆汁淤积患者纳入ICP组(n=20);同期在门诊手术室行终止妊娠术的正常早孕期妇女8例,在计划生育科采用米非司酮+米索前列醇或水囊引产的正常中孕期妇女7例及在产科手术室行剖宫产术正常分娩的晚孕期妇女20例,纳入对照组(n=35)(本研究遵循的程序符合本院人体试验委员会所制定的伦理学标准,得到该委员会批准,分组征得受试对象本人的知情同意,并与之签署临床研究知情同意书)。两组胎盘组织中OATP-A mRNA,OATP-C mRNA测定,采用实时荧光定量PCR法;胎盘组织中有机阴离子转运多肽-A细胞学定位,采用免疫组织化学法。结果①对照组妊娠各期及ICP组胎盘组织中均检出OATP-A mRNA表达,均未发现OATP-C mRNA表达。对照组早、中、晚孕期胎盘组织中,OATP-A mRNA相对量比较,差异无显著意义(P>0.05);ICP组胎盘组织中,OATP-A mRNA相对量高于对照组(40.72±31.46vs.21.54±15.65),差异有显著意义(P<0.05)。②有机阴离子转运多肽-A在对照组晚孕期和ICP组胎盘的表达部位,均为滋养细胞,而在血管内皮细胞无表达。结论胆盐载体OATP-A mRNA在妊娠期肝内胆汁淤积症患者的胎盘组织表达增高,可能是机体对抗胆汁淤积的一种反应。Objective To investigate placental expression of cholate transporter organic anion transporting polypeptide （OATP）-A and -C during normal pregnancy and patient with intrahepatic cholestasis of pregnancy （ICP）. Methods From May 2006 to February 2007, placentas were collected from 20 pregnancy complicated with intrahepatic cholestasis （ICP group）. Meanwhile, 20 women termination pregnancy as well as plan centas from 8 1st trimester and 7 2nd trimester pregnancy were selected into control group （n=35）. RT-PCR was applied for the measurement of placental OATP-A mRNA, OATP-C mRNA expression. This localization of OATP-A was analyzed by immunohistochemistry. The study protocol was approved by the Ethical Review Board of Investigation in Human Being of West China Second University Hospital, Sichuan University. Informed consent was obtained from all participants. Results （1）OATP-A mRNA was detected in placentas in control group of 1st, 2 nd, 3 rd trimester and ICP group placentas, transcript of organic anion transporting polypeptide-C was not detected in these placentas. Placental OATP-A mRNA expression was higher in ICP group than that in control group （40.72 ±31.46 vs. 21.54±15.65, P〈0.05）. No significant difference was found in placental OATP-A mRNA expression among 1st, 2nd and 3rd trimester placentas in control group. （2）organic anion transporting polypeptide-A expression was detected in trophocytes by immunohistochemistry. Conclusion OATP-A mRNA expression is up-regulated in intrahepatic cholestasis of pregnancy placenta, which may represent a compensatory （anticholestatic） mechanism for increased bile acids levels in intrahepatic cholestasis of pregnancy.

关 键 词：妊娠期肝内胆汁淤积症 胎盘 胆盐载体 有机阴离子转运多肽A 有机阴离子转 运多肽-C 

分 类 号：R714.2[医药卫生—妇产科学]