昆虫化学感受蛋白基因的进化分析  

Evolution analysis of the insect chemosensory protein gene family

作　　者：柳晓磊[1] 翁群芳[1] 胡美英[1] 李亚楠[1] 易欣[1] 

机构地区：[1]华南农业大学昆虫毒理研究室/天然农药与化学生物学教育部重点实验室,广州510642

出　　处：《华中农业大学学报》2011年第2期177-181,共5页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(31071713);教育部高等学校博士学科点专项科研基金(20094404110019)

摘　　要：从基因库中调取已完成测序的昆虫化学感受蛋白(chemosensory proteins,CSPs)基因序列和氨基酸序列,共涉及昆虫7个目23个种的59个基因。应用MEGA 5.0形成了这59个基因的系统发育关系树,并计算CSPs基因在昆虫中的核苷酸替换速率。对非同义替换速率和同义替换速率进行比较的结果显示,CSPs基因在昆虫中总体上受到负选择作用。用PAML软件位点-特异模型检测到6个受到正选择作用的位点,其中5个位于功能重要的区域。The phylogenetic trees of the insect chemosensory proteins（CSPs） gene from 59 sequences of CSPs in 23 species under 7 orders published in GeneBank were generated by MEGA 5.0.Complete comparisons of CSPs sequences were used to determine the rates of synonymous（Ks） and nonsynonymous（Kn） codon substitutions and the ratio of Kn/Ks was calculated to estimate of the selection pressure on CSPs genes in insects.The Kn/Ks ratio was far less than 1,suggesting that CSPs may be evolving under negative selection.By the site-specific model in PAML 4.4,6 amino acid sites undergoing positive selection were detected,5 of them were in the important function regions.

关 键 词：昆虫 化学感受蛋白 基因 进化 

分 类 号：Q963[生物学—昆虫学] Q966