马铃薯腐烂茎线虫类毒液过敏原蛋白基因cDNA全长的克隆与序列分析  

Cloning and sequence analysis of a venom allergen-like proteins gene from Ditylenchus destructor on potato in China

作　　者：王秉宇[1,2] 彭德良[2] 黄文坤[2] 彭焕[2] 王高峰[2] 

机构地区：[1]吉林农业大学农学院,长春130118 [2]中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室,北京100193

出　　处：《华中农业大学学报》2011年第2期182-186,共5页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(30871627);国家重大基础研究发展计划(973)项目(2009CB119200)

摘　　要：以马铃薯腐烂茎线虫(Ditylenchus destructor)为材料,用RACE方法,获得了类毒液过敏原蛋白新基因Dd-vap-2的cDNA全长(GenBank登录号为GU370352)。该cDNA全长序列为1 150 bp,包括1个912 bp的完整ORF,编码1个含303个氨基酸的蛋白,其理论分子质量为31.8 ku,等电点pI为6.76。序列比对分析表明,De-vap-2基因含有保守性结构域,属于富含半胱氨酸分泌蛋白(cysteine-rich secretory protein,CRISP)家族成员,N端具有21个氨基酸残基组成的信号肽。系统进化树分析表明,该基因与马铃薯金线虫(Globodera ros-tochiensis)和大豆孢囊线虫(Heterodera glycines)分泌的类毒液过敏原蛋白属于同一支,亲缘关系较近。A new venom allergen-like proteins,named Dd-vap-2,was isolated from Ditylenchus destructor by RT-PCR and RACE.The full length cDNA of Dd-vap-2 consists of 1 150 bp,with a 912 bp ORF encoding a 303 amino acid protein（GenBank accession No.GU370352）.The putative protein with the oretical molecular weight was 31.8 ku in weigh and its pI was 6.76.The sequence analysis indicated that this gene was classified as a member of cysteine-rich secretory protein,CRISP and has a 21 amino acids long signal sequence at N terminal.Phylogenic analysis suggested that Dd-vap-2 had a close homology to Globodera rostochiensis and Heterodera glycines.

关 键 词：马铃薯腐烂茎线虫 类毒液过敏原蛋白基因 富含半胱氨酸分泌蛋白家族 

分 类 号：S432.45[农业科学—植物病理学]