枸杞种质遗传多样性的AFLP分析  

Analysis of genetic diversity for wolfberry germplasms by AFLP technology

作　　者：李彦龙 樊云芳 戴国礼 安巍 曹有龙 

机构地区：[1]国家(宁夏)枸杞工程技术研究中心,宁夏银川750002

出　　处：《中草药》2011年第4期770-773,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30760127)

摘　　要：目的对15份枸杞种质(包括7个种3个变种,5个品种品系)的亲缘关系进行初步研究。方法应用DNA-AFLP分子标记技术和NTSYS类平均法对枸杞种质进行聚类分析。结果 8对引物共扩增出432条带,其中多态性条带为360条,多态性比率达83.3%。NTSYS类平均法聚类结果显示,以0.72、0.79和0.85的相似系数可将全部受试枸杞种质分为9种、5种和3种聚合类群。结论在分子水平上枸杞种间的遗传多样性十分丰富;雄性不育枸杞YX-1与白花枸杞和圆果枸杞亲缘关系较近,而与宁夏枸杞栽培品种的亲缘关系较远;3个变种的聚类结果与传统的形态学分类存在着明显不同。Objective To study the genetic relationships of fifteen wolfberry germplasm,including seven wild species,three varieties,and five cultivars and lines.Methods Data were analyzed by the technology of genomic DNA-AFLP markers and the NTSYS average similarity cluster analysis.Results The statistics showed that 432 bands were amplified using eight pairs of primers,polymorphic bands had account of 360 numbers,and polymorphic rate was 83.3%.All germplasm were classified into nine groups,five groups and three groups as different coefficient of 0.72,0.79,and 0.85.Conclusion This study reveals a diverse basis and their genetic relationship of Chinese wolfberry at the molecular level.The male sterility YX-1 is clustered together with a white flowers wolfberry and a round fruit wolfberry,and YX-1 is more close to the two kinds of wolfberry but farther to the Ningxia wolfberry in the genetic distant.In addition,the classification is obviously different with the traditional morphological one about three variants.

关 键 词：枸杞 遗传多样性 亲缘关系 AFLP 聚类分析 

分 类 号：R282.7[医药卫生—中药学]