谷胱甘肽复方注射液对肝纤维化大鼠肝组织PDGF-B及TGF-β1 mRNA和蛋白表达的影响  

Effect of compound glutathione inosine injection on the expression of platelet-derived growth factor-B and transforming growth factor-β1 in liver tissue of rats with hepatic fibrosis

作　　者：梁跃东[1] 程明亮[1] 李伟[1] 曾经章[1] 吴君[1] 

机构地区：[1]贵阳医学院感染病学教研室,贵阳550004

出　　处：《中国新药杂志》2011年第7期625-628,631,共5页Chinese Journal of New Drugs

摘　　要：目的:研究谷胱甘肽复方注射液(CGII)对猪血清所致免疫性肝纤维化大鼠肝组织中血小板衍生生长因子-B(PDGF-B)及转化生长因子-β1(TGF-β1)mRNA和蛋白表达的影响。方法:建立猪血清诱导免疫性肝纤维化模型成功后,药物治疗6周。实时荧光定量PCR及免疫印迹法检测大鼠肝组织中PDGF-B和TGF-β1 mRNA以及蛋白的表达。结果:在猪血清诱导大鼠肝纤维化模型,CGII(5.4,10.8 mg.kg-1,im)能明显降低肝组织PDGF-B及TGF-β1 mRNA的表达;CGII(2.7,5.4,10.8 mg.kg-1,im)能明显降低肝组织PDGF-B及TGF-β1蛋白的表达。结论:CGII抗大鼠免疫性肝纤维化的作用机制可能与其下调肝组织PDGF-B和TGF-β1 mRNA及蛋白水平的表达有关。Objective： To investigate the effect of compound glutathione inosine injection（CGII） on the expression of platelet-derived growth factor-B（PDGF-B） and transforming growth factor-β1（TGF-β1） in rats with immunological hepatic fibrosis.Methods： Immunological hepatic fibrosis was induced by intraperitoneal injection of porcine serum.Then the rats were treated with CGII for 6 weeks.The mRNA and protein expressions of PDGF-B and TGF-β1 in liver tissue were detected by real-time RT-PCR and Western blotting,respectively.Results： Compared with hepatic fibrosis control,the mRNA levels of PDGF-B and TGF-β1 were significantly reduced in rats treated with CGII（5.4,10.8 mg·kg^-1im）;the protein levels of PDGF-B and TGF-β1 were significantly reduced in rats treated with CGII（2.7,5.4,10.8 mg·kg^-1im）.Conclusion： CGII can inhibit the development of hepatic fibrosis induced by porcine serum in rats.The underlying mechanism might be related with decreasing the expression of PDGF-B and TGF-β1 in the liver.

关 键 词：谷胱甘肽复方注射液 肝纤维化 血小板衍生生长因子-B 转化生长因子-Β1 

分 类 号：R965.1[医药卫生—药理学] R975.5[医药卫生—药学]