微流控芯片测定盐酸金刚烷胺片中的盐酸金刚烷胺  

Determination of Amantadine Hydrochloride in Amantadine Hydrochloride Tablets by Microfluidic Chip Method

作　　者：蔡自由[1] 李永冲[1] 童艳丽[2] 陈缵光[3] 

机构地区：[1]广东食品药品职业学院药学系,广东广州510520 [2]随州职业技术学院医护系,湖北随州441300 [3]中山大学药学院,广东广州510089

出　　处：《分析测试学报》2011年第4期453-456,共4页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20575080和20727006)

摘　　要：建立了微流控芯片非接触电导检测片剂中盐酸金刚烷胺的分析方法。对缓冲液和添加剂的种类及浓度、分离电压、进样时间等进行了优化。实验采用1 mmol/L HAc+2 mmol/L NaAc(pH 4.5)+0.1 mmol/LSDS的缓冲体系,于2.00 kV的分离电压下进样10 s,在1 min内实现了盐酸金刚烷胺的快速检测。优化条件下,盐酸金刚烷胺的线性范围为10～120 mg/L(r=0.999 0),检出限(S/N=3)为0.6 mg/L,加标回收率为97%～98%,相对标准偏差为0.8%～2.2%。方法简便快速,重现性好,适用于盐酸金刚烷胺制剂的快速检测和药品生产质量控制。A new method of microfluidic chip with contactless conductivity detection was established for the determination of amantadine hydrochloride in amantadine hydrochloride tablets. The electro-phoretic parameters, such as the variety and concentration of buffer solution and additive, separation voltage and injection time etc, were investigated. Under the optimal conditions, using 1 mmol/L HAc ＋ 2 mmol/L NaAc （pH 4. 5） ＋ 0. 1 mmol/L SDS as buffer solution system, amantadine hydrochloride was separated and detected in 1 rain at the separation voltage of 2.00 kV and 10 s injection time. The linear dependence of the concentration of amantadine hydrochloride ranged from 10 mg/L to 120 mg/L （ r = 0. 999 0 ）, with a detection limit （ S/N = 3 ） of 0.6 mg/L. The spiked recoveries ranged from 97% to 98% with RSDs of 0. 8% -2.2% . The method was simple, rapid and well reproducible, and could be used for the rapid detection and the quality control of the product.

关 键 词：微流控芯片 非接触电导检测 盐酸金刚烷胺 测定 

分 类 号：O657.1[理学—分析化学] TQ460.72[理学—化学]