类固醇代谢相关基因在大鼠肝再生中表达模式和作用分析  

Expression patterns and action analysis of steroid metabolismasso-ciated genes during rat liver regeneration

作　　者：蔺芳[1] 王书丽[1] 徐存拴 

机构地区：[1]新乡学院生命科学与技术系,河南新乡453000 [2]河南省-科技部共建细胞分化调控重点试验室,河南新乡453000

出　　处：《东北农业大学学报》2011年第3期110-117,共8页Journal of Northeast Agricultural University

基　　金：国家重点基础研究发展计划"973"项目(2006CB708506)

摘　　要：为在基因转录水平了解类固醇代谢相关基因在大鼠肝再生(Liver regeneration,LR)中作用,文章通过搜集网站资料和查阅相关论文等方法获得了参与上述代谢活动的基因,用基因芯片检测了它们在大鼠再生肝中表达情况,初步证实上述基因中83个基因与肝再生相关。肝再生启动(PH后0.5～4h)、G0/G1过渡(PH后4～6h)、细胞增殖(PH后6～66h)、细胞分化和组织结构功能重建(PH后72～168h)等四个阶段起始表达的基因数为48、11、33和3;总表达的基因数为48、41、80和54。表明肝再生相关基因主要在肝再生启动阶段起始表达,在不同阶段发挥作用。它们共表达上调286次,下调286次,表达和功能的相关性分为12类,表明肝再生中细胞生理生化活动多样和复杂。根据上述结果推测,肝再生前期雌激素合成增强;后期胆固醇、胆汁酸合成增强;雄激素、糖皮质激素、孕酮合成几乎在整个肝再生中增强。肝再生早期、前期和后期孕酮和雌激素分解增强;后期胆固醇分解增强;胆汁酸、雄激素、盐皮质激素分解几乎在整个肝再生中增强。To study the action of the genes associated with steroid metabolism during liver regeneration （LR） at transcriptional level.The associated genes mentioned above were obtained by collecting the data of databases and referring to these,and the gene expression changes in the rat regenerating liver were checked by the gene chips.It was found that 83 genes were associated with liver regeneration.The initial and total expressing gene numbers in initiation phase of liver regeneration （0.5-4 h after PH）,G0/G1 transition （4-6 h after PH）,cell proliferation （6-66 h after PH）,cell differentiation and structure-function reconstruction （72-168 h after PH） were 48,11,33,3 and 48,41,80,54,respectively,illustrating that the associated genes mainly were triggered in initiation phase of LR,and worked in different phases.Frequencies of both up-regulation and down-regulation being 286 as well as classification of expression patterns into 12 types showed the diversification and complication of genes expression.It was inferred from the above gene expression changes and patterns that estrogen biosynthesis at prophase and biosynthesis of cholesterol and bile acid at anaphase were augmented;the increases in biosynthesis of androgen,glucocorticoid and progesterone almost during the whole LR;catabolism of progesterone and estrogen at forepart,prophase and anaphase,androgen catabolism at metaphase,and cholesterol catabolism at anaphase were all enhanced;and the catabolism of bile acid and mineralocorticoid remained almost during the whole LR.

关 键 词：部分肝切除 基因芯片 类固醇代谢 肝再生相关基因 

分 类 号：R961[医药卫生—药理学]