壳脂胶囊对NASH小鼠肝组织血红素氧合酶-1表达的影响  

Effects of Kezhi capsule on hepatic expression of heme oxygeanse-1 in mice with non-alcoholic steatohepatitis

作　　者：王荣琦[1] 南月敏[1] 赵素贤[1] 袁丽君[1] 付娜[1] 孔丽[1] 

机构地区：[1]石家庄市河北医科大学第三医院中西医结合肝病科,050051

出　　处：《实用肝脏病杂志》2011年第2期98-100,118,共4页Journal of Practical Hepatology

摘　　要：目的观察壳脂胶囊对非酒精性脂肪性肝炎小鼠肝组织血红素氧合酶-1(HO-1)表达的影响。方法将C57 BL/6J小鼠20只随机分为对照组(采用蛋氨酸-胆碱充足饮食喂养)、模型组[给予高脂、蛋氨酸-胆碱缺乏(MCD)饮食喂养]、壳脂胶囊治疗组和自然恢复组。8周末检测血清转氨酶、瘦素水平,并观察肝组织脂肪变、炎症活动及纤维化程度;定量检测肝组织HO-1 mRNA的表达。结果治疗组动物肝脂肪变及炎症程度较模型组明显减轻,血清瘦素水平和肝组织HO-1表达较模型组明显降低(P<0.05)。结论在高脂、胆碱-蛋氨酸缺乏饮食诱导的NASH小鼠,壳脂胶囊可降低肝组织HO-1表达,阻止NASH的发生发展。Objective To investigate the potential role of Kezhi capsule on the hepatic expression of heme oxygeanse-1（HO-1）in mice with non-alcoholic steatohepatitis（NASH）.Methods Twenty male C57BL/6J mice were randomly divided into four groups：mice fed with methionine-choline adequate diet were controls;The NASH model group were fed with MCD diet;The Kezhi capsule treatment group were fed with MCD diet for four weeks,then plus chitosan and the spontaneous recovery group were fed with methionine-choline adequate diet after four weeks MCD feeding.8 weeks later,blood samples were collected for the detection of serum transaminases and leptin.The histological changes were observed under light microscope and HO-1 mRNA was detected by real-time quantitative PCR.Results Compared with those in MCD group,the hepatic pathological changes were ameliorated,and the hepatic expressions of HO-1 and serum leptin were decreased in Kezhi capsule group（P0.05）.Conclusions Kezhi capsule can down-regulate the expressions of HO-1 and leptin,so it can attenuate hepatic steatosis and necroinflammation in mice with NASH.

关 键 词：非酒精性脂肪性肝炎 血红素氧合酶-1 瘦素 

分 类 号：R285.5[医药卫生—中药学]