环介导等温扩增技术及其在寄生虫学研究中的应用  

Use of loop-mediated isothermal amplification in parasitology research

作　　者：刘航[1] 李彦[1] 杨毅梅[1] 

机构地区：[1]大理学院基础医学院寄生虫教研室,云南大理671000

出　　处：《中国病原生物学杂志》2011年第3期236-237,216,共3页Journal of Pathogen Biology

基　　金：国家自然科学基金项目(No.30860252)

摘　　要：环介导等温扩增(LAMP)技术是利用2对特殊设计的引物,一种具有链置换活性的DNA聚合酶,在恒温条件下特异、高效地扩增DNA的新技术,扩增产物为一系列反向重复的靶序列构成的茎环结构和多环花椰菜样结构的DNA片段混合物,电泳图谱呈阶梯式带状分布,反应伴有白色的副产物焦磷酸镁沉淀产生,可通过肉眼定性判断反应结果。作为一种新的核酸扩增技术,已被应用于疟疾、血吸虫等寄生虫的研究中。LAMP方法敏感、特异,简便,在寄生虫学研究中有广阔的应用前景。A novel method of nucleic acid amplification,termed loop-mediated isothermal amplification（LAMP）,employs two pairs of specially designed primers and DNA polymerase with strand displacement activity.LAMP can efficiently amplify DNA with high specificity under isothermal conditions.The final products are stem-loop DNA with several inverted repeats of the target sequence and cauliflower-like structures with multiple loops.DNA synthesis is accompanied by a byproduct in the form of a white precipitate of magnesium pyrophosphate,providing a visible result.As a new technique for nucleic acid amplification,LAMP has been used in the study of parasitic diseases such as malaria and schistosomiasis.Research has shown that LAMP is sensitive,specific,and easy to perform.LAMP has a range of potential applications in parasitology research.

关 键 词：环介导等温扩增 寄生虫学 综述 

分 类 号：R38[医药卫生—医学寄生虫学]