异位子宫内膜间质细胞的分离培养及MMP2 9的表达和调节  

Determination of Matrix Metalloproteinase2 9 in Cultured Endometriotic Stromal Cells

作　　者：王丽[1] 郭雪霏 

机构地区：[1]中国人民解放军第305医院妇产科,北京100017

出　　处：《河北医学》2011年第3期288-290,共3页Hebei Medicine

摘　　要：目的:建立异位子宫内膜间质细胞分离培养方法,探讨异位子宫内膜间质细胞MMP2、MMP9的表达及调节。方法:收集16例子宫内膜异位症患者的异位内膜进行体外细胞分离及培养,用含肿瘤坏死因子α(TNF-α)的培养液诱导培养24小时,设立对照组,应用免疫组化及明胶酶谱法检测各组细胞中MMP2、MMP9蛋白的表达变化及其培养上清液中MMP2、MMP9的含量。结果:异位子宫内膜细胞中持续表达MMP2、MMP9,与对照组相比,异位内膜间质细胞经TNF-α作用后MMP9表达增高,培养上清液中MMP9的含量也显著增加。结论:成功分离培养了16例异位内膜间质细胞;异位内膜间质细胞持续分泌MMP2、MMP9;TNF-α可诱导MMP9的表达升高,这可能与异位子宫内膜间质细胞的侵袭转移密切相关。Objective： To investigate the clinical significance of matrix metalloproteinase 2、9（MMP2、MMP9） in endometriosis.Method： The expression of MMP2 MMP9 protein were determined by immunohistochemical method and geltin in cultured endometriotic cells with median（controls） or with tumor necrosis factor-α（TNF-α）.The endometriotic tissues were sourced from 16 patients with endometriosis.Meanwhile the MMP2、MMP9 protein content of cultured supematent was also measured.Result： Endometriotic stromal cells continualy expressed MMP2、MMP9,after exposured to TNF-α,the expression of MMP9 protein in the endometriotic cells was statisticaly higher compared with control group（P〈0.05）,so was the MMP9 protein content of supermatent.However,we did not find difference in MMP2 between the two groups.Conclusion： TNF-α can up-regulate the expression of MMP-9 in endometriotic cells,which may be related to the development of endometriosis.

关 键 词：子宫内膜异位症 细胞培养 基质金属蛋白酶 肿瘤坏死因子-Α 

分 类 号：R711.71[医药卫生—妇产科学]