新天然来源抗生素-抗霉素A_(18)的抗肿瘤活性及其产生菌I07A-01824的生物学特征  

Preliminary studies on cytotoxicity of antimycin A_(18) and the biological characteristics of its producing strain I07A-01824

作　　者：闫蕾蕾[1,2] 韩宁宁[1,3] 何琪杨[1] 陈杰[1] 阳素娥[4] 刘少伟[1] 陶玲[1] 郭琳[1] 靳婧[1] 余利岩[1] 张玉琴[1] 孙承航[1] 

机构地区：[1]中国医学科学院北京协和医学院医药生物技术研究所,北京100050 [2]河北大学生命科学学院,保定071002 [3]中国兽医药品监察所,北京100081 [4]桂林医学院,桂林541004

出　　处：《中国抗生素杂志》2011年第4期269-275,279,共8页Chinese Journal of Antibiotics

基　　金：科技部十一五"重大新药创制"科技重大专项-综合性新药研究开发技术大平台(2009zx09301-003);国家科技重大专项-创新微生物药物高效筛选与发现技术平台研究2009ZX09302-004的资助

摘　　要：目的检测抗霉素A18的体外抗肿瘤活性并对其产生菌I07A-01824的形态及培养特征,生理生化特征等进行研究。方法用MTT法检测抗霉素A18的肿瘤细胞生长抑制作用,用流式细胞术测定其对细胞周期的影响;按分类学常规方法研究菌株I07A-01824的形态及部分生理生化特征。结果抗霉素A18能够抑制人肝癌细胞HepG2、人口腔上皮癌细胞KB的生长;这两个细胞株均被阻滞在细胞周期的S期。菌株I07A-01824的形态及培养特征与已知种——黄白链霉菌(Streptomyces albidoflavus)一致。结论红树林内生放线菌是新抗生素的重要来源。Objective Preliminary studies on the in vitro cytotoxicity of antimycin A18, a novel antibiotic produced by strain I07A-01824 isolated from mangrove plant and the morphological, cultural and physiological characteristics of strain I07A-01824. Methods The cell growth inhibitory activities of antimycin A,8 on human hepatic carcinoma cell line HepG2 and human oral epidermoid carcinoma cell line KB were detected by MTT assay. Effects on cell cycle of the two cell lines were examined by flow cytometry. Morphological, cultural and physiological characteristics of I07A-01824 were carried out by classic taxonomic methods for actinomycetes. Results Antimycin A18 can inhibit the growth of both HepG2 cells and KB cells in low concentrations. The arrest at S phase was detected by flow cytometry in both HepG2 and KB cells after antimycin A18 treatment with different concentrations. The morphological, cultural and physiological characteristics of strain I07A-01824 were the same as a known species, Streptomyces albidoflavus, but can produce novel antimycin group antibiotics. Conclusion Actinomycetes from the mangrove ecosystem were good resources for novel antibiotics discovery.

关 键 词：红树林 内生放线菌 抗霉素A18 黄白链霉菌 细胞毒活性 

分 类 号：R979.14[医药卫生—药品]