瓶花木内生真菌C22发酵液中生物活性成分的分离、纯化与鉴定  

Separation,purification and structural elucidation of the bio-active components from fermentation broth of endophytic fungus C22 from Scyphiphora hydrophyllacea

作　　者：曾艳波[1,2] 戴好富[2] 黄玖利[2] 梅文莉[2] 

机构地区：[1]海南大学园艺园林学院,海口570228 [2]中国热带农业科学院热带生物技术研究所农业部热带作物生物技术重点开放实验室,海口571101

出　　处：《中国抗生素杂志》2011年第4期276-279,共4页Chinese Journal of Antibiotics

基　　金：国家自然科学基金(20862020);海南省普通高等学校研究生创新科研课题(HXWBY2009-01)

摘　　要：目的分离、鉴定红树林植物瓶花木内生真菌C22的活性次生代谢产物。方法利用硅胶柱层析等方法进行分离纯化;利用光谱分析进行结构解析。结果内生真菌C22的活性次生代谢产物为一大环内酯类抗生素布雷菲尔德菌素A和一倍半萜类抗生素木霉菌醇。结论以上两种抗生素对肺癌细胞SMMC-7721、胃癌细胞SGC-7901具有较强的肿瘤细胞毒活性,并且在内生真菌C22中含量较大,因此,红树林植物瓶花木内生真菌菌株C22具有广阔的应用价值和开发前景。Objective To elucidate the structures of the bio-active component isolated from the fermentation broth of endophytic fungus strain C22 from mangrove plant Scyphiphora hydrophyllacea Gaertn. E Methods Silica gel column chromatography was used to separate the bio-active components. Their structures were elucidated by spectroscopic analyses. Results The active components were purified and their structures were elucidated as two antibiotics： brefeldin A and trichodermol. Conclusions Endophytic fungus strain C22 with a large content of these two antibiotics, which showed inhibitory activities towards human hepatoma cell line SMMC-7721 and human gastric cancer cell line SGC-7901, has broad prospects for commercial application.

关 键 词：瓶花木 内生真菌 布雷菲尔德菌素A 木霉菌醇 

分 类 号：R978[医药卫生—药品]