噻重氮苯基脲对文冠果愈伤组织诱导与分化的影响  

Study on Effects of Different Concentrations of TDZ on Callus Induction of Xanthoceras sorbifolia

作　　者：任如意[1] 王书臻[1] 司徒琳莉[1] 纪萍[2] 

机构地区：[1]牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157012 [2]黑龙江省林副特产研究所,黑龙江牡丹江157011

出　　处：《北方园艺》2011年第6期127-129,共3页Northern Horticulture

基　　金：黑龙江省普通高校青年学术骨干计划资助项目(1152G047);牡丹江师范学院大学生创新资助项目

摘　　要：以文冠果茎段和幼嫩叶片为外植体,分别进行了茎段和幼嫩叶片的组织培养试验,研究了附加不同浓度的噻重氮苯基脲(TDZ)愈伤组织和不定芽分化的影响。结果表明:MS+TDZ 0.05 mg/L+6-BA 1.0 mg/L+NAA 0.01 mg/L培养基对幼叶的诱导分化培养效果最好;MS+TDZ 0.02 mg/L+6-BA 1.0 mg/L+NAA 0.02 mg/L培养基对茎段诱导不定芽的效果最好;而TDZ在文冠果生根培养中不起作用。With stem section and younger leaves of Xanthoceras sorbifolia as explants,tested some tissue culture experiments on stem section and younger leaves,studied the effects of different concentrations of TDZ on callus induction and adventitious bud differentiation.The results showed that MS＋TDZ 0.05 mg/L＋6-BA 1.0 mg/L＋NAA 0.01 mg/L medium had the best effect on differentiation culture for younger leaves,MS＋TDZ 0.02 mg/L 6-BA 1.0 mg/L＋NAA 0.02 mg/L medium had the best effect on adventitious bud for stem section;but TDZ had no effect on rooting culture Xanthoceras sorbifolia.

关 键 词：文冠果 TDZ 愈伤组织 

分 类 号：S667.9[农业科学—果树学]