外伤性PVR状态大鼠视网膜前膜c-fos和c-jun的表达  

Dynamic expression of c-fos and c-jun in epiretinal membrances in experimentally induced proliferative vitreoretinopathy

作　　者：陶雪[1] 艾明[1] 邢怡桥[1] 项奕[1] 贺涛[1] 江双红[1] 

机构地区：[1]武汉大学人民医院眼科中心,武汉430060

出　　处：《医药论坛杂志》2011年第2期7-9,共3页Journal of Medical Forum

基　　金：湖北省科技攻关计划项目基金资助(302131154)

摘　　要：目的观察与探讨实验性外伤致大鼠增殖性玻璃体视网膜病变时,大鼠视网膜前膜中c-fos和c-jun的表达及其相关性。方法采用S-P法在不同时间点对外伤性PVR大鼠视网膜前膜做c-fos和c-jun免疫组织化学染色,染色结果行图象分析。结果实验中c-fos和c-jun表达阳性细胞率和平均光密度值先升高后稍降低,14d组和21d组阳性率最高,与7d组比较有显著性差异,c-fos和c-jun表达之间有显著相关性。结论 c-fos和c-jun参与PVR视网膜前膜细胞增殖的调控,两者的变化呈高度相关性,是促进PVR细胞增埴过程中一个重要的早期分子事件,为用基因手段防治PVR以及治疗时间的选择提供实验依据。Objective To observe and investigate the expression and their correlation of c-fos and c-jun in the formation of epiretinal membranes of traumatic proliferative vitreoretinopathy（PVR）.Methods S-P technique were applied for immunocytochemical staining of epiretinal membranes of traumatic PVR with c-fos and c-jun antibody.The staining results were analyzed with image analysis system.Results The positive rate and average OD of c-fos and c-jun increased at first and then decreased after trauma.Compared with 7 days group,the positive rate in 14 days group and 21 days group were higher.There was significant correlation among c-fos and c-jun protein expression.Conclusions c-fos and c-jun play important roles in the regulation of proliferative cells in epiretinal membranes of traumatic PVR,and have high correlation with each other.It provides experimental basis for preventing or treating PVR by gene technique and the choice of therapy time.

关 键 词：增殖性玻璃体视网膜病变 细胞增殖 原癌基因 免疫组织化学 

分 类 号：R332[医药卫生—人体生理学]