液相色谱-串联三重四极杆质谱测定牛奶中的氯霉素残留量  

Determination Chloramphenicol Residues in Milk by LC-MS-MS

作　　者：田艳玲[1] 王浩[1] 

机构地区：[1]国家食品质量安全监督检验中心,北京100094

出　　处：《食品研究与开发》2011年第3期125-127,共3页Food Research and Development

摘　　要：建立牛奶中氯霉素残留的高效液相色谱-串联四极杆质谱联用测定方法。该方法经乙腈提取样品,以MGⅢ-C18柱(2.1×150 mm,1.8μm)分离,流动相为水溶液和乙腈(体积比为65∶35),电喷雾负离子MRM模式检测。该方法的检出限0.07μg/L,线性范围0.05μg/L^20.0μg/L,加标回收率98.5%～110.8%,相对标准偏差为3.57%。This thesis developed a LC-MS-MS method for Chloramphenicol residues in milk.The mass spectrometer was operated in the negative ion mode using multiple reaction monitoring.In the method,A sample was extracted with acetonitrile,Chromatographic separation was carried out on a MGⅢ-C18 column（2.1×150 mm,1.8 μm） with eluthicsion of acetonitrile-water（35∶65 by volume）.The limit of detection is 0.07 μg/L,The linear plots were 0.05 μg/L-20.0 μg/L.Overall recoveries were 98.5 %-110.8 %,and RSD value was 3.57 %.

关 键 词：液质联用仪 氯霉素 牛奶 残留 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]