不同家蚕品种对桑叶中1-脱氧野尻霉素的富集能力调查  

An Investigation on Enrichment Ability of 1-Deoxynojirimycin from Mulberry Leaves by Various Bombyx mori Varieties

作　　者：杨利利[1] 焦锋[1] 杜明[1] 潘璐璐[1] 苏超[1] 宋新华[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《蚕业科学》2011年第2期362-366,共5页ACTA SERICOLOGICA SINICA

基　　金：现代农业产业技术体系建设专项(蚕桑)

摘　　要：家蚕体内的1-脱氧野尻霉素(DNJ)来源于桑叶,为筛选对食下桑叶中DNJ有较强富集能力的家蚕品种用于功能性食品及药物的开发,以18个家蚕品种和6个桑树品种为材料,采用反相高效液相色谱-紫外检测法测定供试家蚕品种5龄第3天幼虫制备全蚕粉中的DNJ含量。不同地理系统家蚕品种对食下桑叶中DNJ的富集能力存在明显差异,其中日系品种与欧系品种对桑叶中DNJ的富集能力有显著差异(前者DNJ质量分数平均为0.3007%,后者为0.2469%),中系品种的DNJ质量分数平均为0.288 0%,与日系品种与欧系品种之间无显著差异;不同眠性(三眠与四眠)家蚕品种间对桑叶中DNJ的富集能力差异不明显。18个供试家蚕品种中,中系品种797对桑叶中DNJ的富集作用最强,其全蚕粉中的DNJ质量分数达0.4345%。选择6个家蚕品种分别取食不同桑树品种的桑叶,分析全蚕粉中的DNJ含量和桑叶中DNJ含量的相关性,其相关系数为0.826 0,全蚕粉中DNJ含量最高的家蚕品种与桑品种组合为家蚕乌2与陕桑403,其次为家蚕武功土种与甜桑、家蚕798与陕桑403。1-Deoxynojirimycin（DNJ） in silkworm（Bombyx mori） is derived from mulberry leaves.In order to screen silkworm varieties with better enrichment ability from the forage mulberry leaves for development of functional food and medicine,18 silkworm and 6 mulberry varieties were used as the materials for determining DNJ contents in whole body powder of day 3 silkworm larvae of the 5th instar by reverse-phase high-performance liquid chromatography and ultraviolet spectrometry.It was found that silkworm varieties of different geographical strains had remarkable difference in their enrichment ability to DNJ from forage mulberry leaves.Silkworm varieties of Japanese and European strains had significantly different enrichment ability to DNJ from forage mulberry leaves（DNJ mass fraction of the former was 0.300 7% and that of the latter was 0.246 9%）.The average DNJ mass fraction in silkworm varieties of Chinese strain was 0.288 0%,having no significant difference with those of Japanese and European strains.Silkworm varieties of different moltinisms（trimolter or tetramoulter） had no remarkable difference in their enrichment ability to DNJ from forage mulberry leaves.Among the 18 silkworm varieties tested,silkworm variety 797 of Chinese strain had the best enrichment result to DNJ from mulberry leaves.DNJ mass fraction in the whole body powder reached 0.434 5%.The correlation between DNJ mass fraction in the whole body powder and DNJ content in mulberry leaves were analyzed by feeding larvae of 6 silkworm varieties with mulberry leaves of different varieties.The result showed that the correlation coefficient was 0.826 0.The combination with the highest DNJ content in the whole body powder was silkworm variety Wu 2 and mulberry variety Shansang 403,followed by silkworm variety Wugong-tuzhong and mulberry variety Tiansang,and silkworm variety 798 and mulberry variety Shansang 403.

关 键 词：家蚕 1-脱氧野尻霉素 蚕品种 桑品种 富集 

分 类 号：S881.2[农业科学—特种经济动物饲养]