1株鸭源Ⅰ类新城疫病毒分离株F和HN基因的分子特性分析  

Molecular characterization of F and HN genes of class Ⅰ Newcastle disease virus in China

作　　者：冯莉莉[1,2] 刘华雷[2] 郑东霞[2] 赵云玲[2] 张维[2] 张乐萃[1] 王志亮[2] 

机构地区：[1]青岛农业大学动物科技学院,山东青岛266109 [2]中国动物卫生与流行病学中心,山东青岛266032

出　　处：《畜牧与兽医》2011年第4期9-14,共6页Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金资助(30901079);农业部动物疫情监测与防治专项

摘　　要：采用RT-PCR技术对Ⅰ类新城疫病毒(NDV)09-014分离株完整的融合蛋白(F)基因和血凝素-神经氨酸酶(HN)基因进行了扩增和遗传进化分析。F基因的序列测定结果表明:该分离株F基因全长为1 792 bp,可编码553个氨基酸,裂解位点的氨基酸组成为112E-R-Q-E-R-L117,具有典型的新城疫弱毒株特征。同源性分析表明本分离株的F基因与Ⅰ类新城疫病毒代表毒株之间核苷酸的同源性为93%～95.2%,而与Ⅱ类新城疫病毒代表毒株的同源性较低,介于70.6%～72.4%。HN基因的序列测定结果表明:HN基因全长2 001 bp,可编码616个氨基酸,同源性分析表明本分离株的HN基因与Ⅰ类新城疫病毒代表毒株之间核苷酸的同源性在92.7%～94.7%之间,而与Ⅱ类新城疫病毒同源性较低,为70.7%～71.5%。根据完整的F基因和HN基因构建的遗传进化树均表明:本分离株在分类地位上属于Ⅰ类新城疫病毒基因3型,因此Ⅰ类新城疫病毒的F基因和HN基因具有相似的进化速率。The F and HN genes of Newcastle disease virus （NDV） strain NDV09-014, isolated from domestic duck in China, were amplified by RT-PCR and sequenced. The complete F gene was composed of 1 792 nucleotides, encoding 553 amino acids. The motif of the cleavage site of the fusion protein was ^112E-R-Q-E-R-L^117, which is typical of lentogenic NDV strains. Homology analysis of F gene revealed that sequence similarities among class I NDV isolates varied from 93% and 95. 2% , and showed relative lower identities between this isolate and class II NDV strains, 70. 6% to 72. 4%. The full length of HN gene comprised of 2001 nucleotides, encoding 616 amino acids. Ho- mology analysis of HN gene revealed that sequence similarities among class I NDV isolates varied from 92. 7% to 94. 7% , and 70. 7% to 71. 5% of identities between this isolate and class II NDV strains. Phylogenetic analysis based on the complete F and HN gene sequences showed that the NDV09-014 isolate was grouped into genotype 3 in class I . It suggested that the evolutionary rates in F and HN genes of class I were similar.

关 键 词：新城疫病毒 I类 融合蛋白 血凝素-神经氨酸酶蛋白 分子特性 

分 类 号：S852.65[农业科学—基础兽医学]