强力霉素人工抗原的合成与抗体制备  

Artificial Antigen Synthesis and Antibody Preparation of Doxycycline

作　　者：甘金华[1] 邓薇[1] 李进平[2] 艾晓辉[1] 

机构地区：[1]中国水产科学研究院长江水产研究所,湖北荆州434000 [2]武汉纺织大学环境与城建学院,湖北武汉430073

出　　处：《食品与生物技术学报》2011年第2期316-320,共5页Journal of Food Science and Biotechnology

基　　金：农业部公益性行业专项项目(200803013)

摘　　要：采用改进的碳二亚胺两步法将强力霉素半抗原与载体蛋白BSA连接制备强力霉素-牛血清白蛋白(BSA-DC)人工免疫抗原,并用同样方法将强力霉素与载体蛋白OVA连接制备人强力霉素-卵清白蛋白(OVA-DC)人工包被抗原。经紫外扫描分析和动物免疫试验证实:强力霉素人工抗原合成成功,强力霉素与BSA的结合比为3∶1,经动物免疫试验所得抗血清效价为2.048×106,完全能够满足强力霉素残留的ELISA检测要求。In this study,the improved two-step carbodiimide method was used to connecte doxycycline hapten with carrier protein BSA,and doxycycline with the carrier protein OVA to prepare the artificial antigen of doxycycline（BSA-DC） and coating antigen（OVA-DC）,respectively.UV scanning analysis and animal immune tests confirm the success in preparation of artificial antigen,and the combination ratio of doxycycline and BSA is 3∶1 by calculating.At the same time,the results showed that high titers of 2.048×106 anti-DC serum were produced by BSA-DC immunized rabbit.It provided a necessary condition for ELISA detecting doxycycline residues.

关 键 词：强力霉素 人工抗原 抗体 合成 制备 

分 类 号：S859.84[农业科学—临床兽医学]