鹅流感病毒2/96-H_5N_1亚型毒株RNA_(1-3)及RNA_5节段核苷酸全序测定  

The complete nucleotide sequences of A/Goose/Guangdong/2/96(H_5N_1) virus RNA segment 1 3 and 5

作　　者：郭元吉[1] 王敏[1] 郭俊峰[1] 董婕[1] 张烨[1] 

机构地区：[1]中国预防医学科学院病毒学研究所,北京100052

出　　处：《中华实验和临床病毒学杂志》1999年第3期205-208,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　明确广东鹅流感病毒２９６Ｈ５ Ｎ１ 亚型毒株ＲＮＡ１３ 和ＲＮＡ５ 节段核苷酸全序列及其所编码蛋白的氨基酸序列，以及这些基因节段与香港禽流感病毒１５６９７Ｈ５ Ｎ１ 亚型毒株相应节段间的关系。方法　病毒粒ＲＮＡ经逆转录合成ｃＤＮＡ，经聚合酶链反应（ＰＣＲ） 扩增，产物纯化，采用双脱氧链末端终止法进行核苷酸序列测定。结果　广东鹅流感病毒２９６Ｈ５ Ｎ１ 亚型毒株ＲＮＡ１３ 和ＲＮＡ５ 节段长度分别为２３４１，２ ３４１ ，２ ２３３ 和１５６５ 个核苷酸。它们分别编码ＰＢ２（ 含７５９ 个氨基酸），ＰＢ１（ 含７５７个氨基酸） ，ＰＡ（ 含７１６ 个氨基酸） 和ＮＰ蛋白（ 含４９８ 个核苷酸） 。这些蛋白与香港禽流感病毒１５６９７Ｈ５ Ｎ１ 亚型毒株相应蛋白氨基酸序列的同源性分别为９６－４％ ，９７－２％ ，９７－３ ％ 和９７－０％ 。结论　本毒株ＲＮＡ１３ 和ＲＮＡ５ 节段长度分别为２ ３４１，２ ３４１，２ ２３３ 和１ ５６５ 个核苷酸，它们与香港１５６９７Ｈ５ Ｎ１Objective To determine the nucleotide and amino acid sequences of PB 2, PB 1, PA and NP genes and compared them with sequences of A/HK/156/97(H 5N 1) virus for revealing the relationship between A/Googs/Guangdong/2/96(H 5N 1) and A/HK/156/97(H 5N 1) viruses.Methods Virion RNA was transcribed into cDNA by reverse transcriptase, cDNA amplified by PCR, the productions of PCR were purified. Afterward, RNA sequence analysis was performed by the dideoxynucleotide chain termination method, using synthetic oligodeoxynucleotide primers. Results The lengths of A/Goose/Guangdong/2/96(H 5N 1) virus RNA segment 1 3 and 5 contain 2 341, 2 341, 2 233 and 1 565 nucleotides, respectively. They encode for PB 2 (759 amino acids), PB 1 (757 amino acids), PA (716 amino acids) and NP (498 amino acids) proteins. The homologies of amino acid sequences of PB 2, PB 1, PA and NP proteins between A/Goose/Guangdong/2/96 (H 5N 1) and A/HK/156/97 (H 5N 1) virus are 96.4%, 97.2%, 97.3% and 97.0%, respectively. Conclusion The lengths of RNA segment 1 3 and 5 of Goose strain are 2 341, 2 341, 2 233 and 1 565 nucleotides, respectively. The nucleotide sequences of these genes are distinguish able from those of Hong Kong virus.

关 键 词：鹅流感病毒 RNA1-3 核苷酸全序列 HK97-156 

分 类 号：R373.13[医药卫生—病原生物学] R446.5[医药卫生—基础医学]