从中国东北地区蚊标本首次分离到新亚型Colti病毒  

New subtype of coltivirus isolated from mosquitoes in the northeast part of China

作　　者：陶三菊[1] 蔡增林 杨冬荣[1] 王焕琴[1] 刘琴芝[1] 范永星 范秀娟 陈伯权[1] 

机构地区：[1]中国预防医学科学院病毒学研究所,北京100052 [2]沈阳军区医学研究所

出　　处：《中华实验和临床病毒学杂志》1999年第3期228-230,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　从我国东北地区媒介蚊分离新虫媒病毒。方法　采用敏感的Ｃ６３６ 和ＢＨＫ２１细胞分离病毒。结果　从６８ 份蚊标本中分离到两株Ｃｏｌｔｉ 病毒，经间接免疫荧光试验观察到新分离株与已知Ｃｏｌｔｉ 病毒ＴＲＴ２ 株免疫腹水呈阳性反应；ＰＡＧＥ显示新分离株均为１２ 节段双链ＲＮＡ，其电泳带型为６５１，与ＴＲＴ２ 株（６６） 明显不同。组织培养交叉中和试验观察到新分离株不能被已知Ｃｏｌｔｉ 病毒ＴＲＴ２ 株的免疫腹水中和，同样，新分离株的免疫血清亦不能中和ＴＲＴ２ 株。结论　从我国东北地区首次分离到的Ｃｏｌｔｉ 病毒可能为我国ＣｏｌｔｉObjective The purpose of this study was to isolate new arbovirus from mosquitoes collected from the northeast part of China during the summer of 1996.Methods The cell used for isolation were C6/36 and BHK 21 cells.Results Two strains (NE97 12 and NE97 31) of coltivirus were isolated among 68 mosquito specimens. The viruses caused cytopathogenic effects on C6/36 cell, but not on BHK 21 cells. They were resistant to both 5 IDU and ether, but sensitive to pH3.0 and temperature at 56℃ for 30 minutes. IFA of new isolates with mouse ascitic fluid anti coltivirus TRT2 strain showed positive reaction. Polyacrylamide gel electrophoresis (PAGE) of two isolates showed 12 segments of double stranded(ds) RNA and PAGE profiles were 6 5 1, which were different from that of coltivirus TRT2 strain(6 6). There was no cross neutralization between new isolates and TRT2 strain by tissue culture cross neutralization test.Conclusion The results indicated while new isolates were serologically related to TRT2 strain of coltivirus by IFA, the neutralization test suggests that it represents a distinct serotype. These new isolates isolated firstly from the northeast part of China belong to a new subtype of coltivirus.

关 键 词：COLTI病毒 呼肠孤病毒科 蚊 病毒学 RNA 

分 类 号：R373.9[医药卫生—病原生物学]