线探针核酸杂交分析贵州地区HCV感染株基因型  

Analysis of hepatitis C virus genotypes in Guizhou area, using second generation line probe assay

作　　者：丁静娟[1] 李媛媛[1] 田苗[1] 鲍丽雅[1] 丁一生[1] 李佳[1,2] CarineDallo 

机构地区：[1]贵阳医学院附属医院传染科 [2]遵义医学院附属医院传染科 [3]比利时InnoGenetics公司研究部

出　　处：《中华实验和临床病毒学杂志》1999年第3期243-246,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　分析贵州地区丙型肝炎病毒（ＨＣＶ）感染株的基因型。方法　用第二代线探针核酸杂交方法，对９８ 例ＨＣＶ感染者血清进行基因分型。结果　１２ 例献血员血清，均为ＨＣＶ１ｂ 型；１５ 例血液病患者中，１４ 例（９３－３％ ）为ＨＣＶ１ｂ ，１ 例（６－７％ ）为ＨＣＶ１ｂ ＋ ２ 型。慢性丙肝患者３７ 例中，ＨＣＶ１ｂ 型３４例（９１－９％ ） ，混合感染基因型３ 例（ＨＣＶ１ａ ＋ １ｂ 、ＨＣＶ１ｂ ＋ ２ 、ＨＣＶ１ａ ．１ｂ ＋ ２ａ２ｃ 各１ 例）。静脉药瘾者３４ 例，其中ＨＣＶ１ｂ 感染１３ 例（３８－２％ ） ，ＨＣＶ６ａ１０ 例（２９－４％ ） ，ＨＣＶ３ｂ４ 例（１１－８％ ） ，ＨＣＶ３ａ１ 例（２－９ ％） ，混合感染６ 例（１７－６ ％） ，大多为ＨＣＶ２ 复合其他基因型。结论　贵州地区ＨＣＶ感染者中，以ＨＣＶ１ｂ 基因型为主，其次为ＨＣＶ６ａ ，尚存在ＨＣＶ３ａ 和ＨＣＶ３ｂ 。其中ＨＣＶ３ａ、３ｂ 、６ａ ，在本地区系首次报告，但主要存在于静脉药瘾者中。虽然有混合感染基因型的存在。Objective To analyse the frequency of various hepatitis C virus genotypes in different populations with HCV infection from Guizhou area.Methods Ninty eight HCV isolates obtained by using RT nested PCR from 37 patients with chronic hepatitis C (PHCs), 15 patients with hemopathy (PHs), 12 blood donors (BDs) and 34 intravenous drug users (IVDUs) were genotyped by using the second generation line probe assay. Results Among 37 HCV isolates from PHCs, there were 34 (91.9%) genotype 1b, 3 (8.1%) mixted genotypes (1 for 1a+1b, 1 for 1b+2, 1 for 1a+1b+2a/2c, respectively). Among 15 PHs, 14 (93.3%) were type 1b, 1(6.7%) were type 1b+2, among 12 BDs, all(100.0%) were type 1b. Among 34 IVDUs, 13(38.2%) were type 1b,10(29.4%) were type 6a, 1(2.9%) was type 3a, 4(11.8%) were type 3b and 6(17.6%) were mixted types(2 for 1a+2, 1 for 1a、1b+2, 1 for 1b+3b, 1 for 3b+2, 1 for 1b+6a, respectively).Conclusion The results showed that HCV subtypes 1a. 1b, 2a/2c, 3a, 3b and 6a belonging to 4 HCV genotypes(1,2,3,6) were found from different populations in Guizhou, of these, 3a, 3b and 6a were firstly reported. HCV 1b was commonly found in BDs, PHs and PHCs and its frequency was above 90.0%, 3a, 3b, 6a were only found in IVDUs and their total frequency was above 50%.

关 键 词：丙型肝炎病毒 基因型 线探针 

分 类 号：R373.21[医药卫生—病原生物学]