1994～1997年深圳地区流感病毒活动及H3N2亚型毒株HA1基因演变特点  

The feature of influenza virus activity and genetic characterization of HA1 gene of influenza A H3N2 viruses isolated from 1994～1997 in Shenzhen

作　　者：程小雯[1] 李良成[1] 何建凡[1] 

机构地区：[1]深圳市卫生防疫站,580020

出　　处：《中华实验和临床病毒学杂志》1999年第3期259-261,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　了解近几年流感病毒在深圳地区活动的特点及甲３（Ｈ３Ｎ２） 亚型毒株ＨＡ１ 基因演变概况。方法　病毒分离采用常规的鸡胚双腔接种，毒株检定用常量半加敏ＨＩ测定。新鲜收获含病毒粒的鸡胚尿囊液用来提取ＲＮＡ，经逆转录合成ｃＤＮＡ，经聚合酶链反应（ＰＣＲ） 扩增，产物纯化，采用双脱氧链末端终止法进行核苷酸序列测定。结果　近几年来深圳地区流感活动概况与全国情况相一致：在人群中仍同时流行Ｈ３Ｎ２，Ｈ１Ｎ１ 亚型和乙型毒株，当甲型毒株活动减弱时，乙型毒株活动就增强，反之，甲型毒株增强时，乙型毒株就减弱。随着时间的推移，Ｈ３Ｎ２ 亚型毒株ＨＡ１ 基因不断地发生点突变，这种突变严重受人群免疫压力所影响，１９９６ 年的毒株与１９９５ 的毒株相比，不仅氨基酸替换点中多数是位于抗原决定簇区或受体结合部位上，并增加两个糖基化位点，故导致Ｈ３Ｎ２ 毒株於１９９６ 年活动明显增强。结论　近来在深圳地区人群中仍同时流行着Ｈ３Ｎ２，Ｈ１Ｎ１ 亚型和乙型流感病毒。然而，不同年其优势毒株是不一样的。１９９６ 年Ｈ３Ｎ２ 毒株活动增强是由于其ＨＡ１ 区氨基酸序列发生替换所造成。Objective To understand the feature of influenza virus activity and genetic characterization of HA1 gene of influenza A H3N2 viruses isolated in recently years in Shenzhen. Methods The specimens were inoculated into amniotic and allantoic cavities of the embryonated chicken eggs for viral isolation. The isolates were typed with HI test. Virion RNA extracted from allantoic fluid by Rneasy RNA extraction kit was used for RT PCR amplification. Afterward, the product of PCR was purified with purification kit. RNA sequence analysis was performed by Sanger dideoxy mediated nucleotide chain termination method.Results Influenza A H3N2, H1N1 and B viruses were cocirculating in men in Shenzhen in recent years. However, the extent of their activities was not the same in a single year. The influenza B virus was the predominant strain in 1994 and 1997, but the predominant strain was H3N2 virus in 1996. The genetic analysis showed that the substitution of amino acids occured year by year and was mainly located at antigenic determinants or receptor binding site on HA1 domain of H3N2 viruses.Conclusion Influenza A H3N2, H1N1 and B viruses were still co circulating in men in Shenzhen in recent years, the predominant strain of influenza virus was different in different years. An increase of influenza A H3N2 virus activity in 1996 was due to the substitution of amino acids sequences on viral HA1 domain.

关 键 词：流感病毒 H3N2亚型 病毒基因 

分 类 号：R373.13[医药卫生—病原生物学]