IL-4对治疗前后亚急性重型病毒性肝炎患者PBMCs TNF-α和IL-1α mRNA表达的影响  

Influence of IL 4 on the expression of TNF α and IL 1α mRNA by PBMCs from patients with subfulminant viral hepatitis before and after treatment

作　　者：石裕明[1] 张立煌[2] 孙永良[2] 方海林[2] 何南祥[2] 刘克洲[2] 李敏伟[2] 姚航平[2] 吴婉芬[1] 

机构地区：[1]广州市第八人民医院传染病研究所,510060 [2]浙江医科大学传染病研究所

出　　处：《中华实验和临床病毒学杂志》1999年第3期269-273,共5页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　了解白介素（ＩＬ）４ 对重型病毒性肝炎患者外周血单个核细胞（ＰＢＭＣｓ） 中肿瘤坏死因子（ＴＮＦ）α和ＩＬ１αｍＲＮＡ的表达的影响。方法　ＴＮＦα和ＩＬ１αｍＲＮＡ的表达水平采用半定量逆转录聚合酶链反应（ＲＴＰＣＲ） 进行检测。结果　ＩＬ４ 均以剂量依赖的方式抑制治疗前、后亚急性重型肝炎患者ＰＢＭＣｓ ＴＮＦα和ＩＬ１αｍＲＮＡ的表达，在发病初期，ＩＬ４ 于１ ０００Ｕｍｌ 时接近最大抑制效应；而恢复期患者，ＩＬ４ 于１００Ｕｍｌ 时即接近最大抑制效应，剂量－ 反应曲线明显左移。如以１００Ｕｍｌ 的ＩＬ４ 处理ＰＢＭＣｓ，恢复期亚急性重型肝炎患者ＰＢＭＣｓ ＴＮＦα和ＩＬ１αｍＲＮＡ的抑制率接近５０％ 。另外还发现，在发病初期，ＩＬ４ 对内毒素血症和ＨＢｅＡｇ 阳性患者ＰＢＭＣｓＴＮＦα和ＩＬ１αｍＲＮＡ表达的抑制作用低于阴性的患者。结论　ＩＬ４ 对发病初期患者ＰＢＭＣＴＮＦα和ＩＬ１αｍＲＮＡ 表达的抑制作用明显低于恢复期患者。Objective The aim of this study was to compare the influence of IL 4 on the expression of TNF α and IL 1α mRNA by PBMCs from patients with subfulminant viral hepatitis(SFH) before and after IL 4 treatment. Methods The expression levels of TNF α and IL 1α mRNA by PBMCs were assessed by semiquantitative RT PCR.Results IL 4 suppressed the expression of both TNF α and IL 1 αmRNA by PBMCs from SFH patients before and after treatment dose dependently. However, their dose response curves showed significant differences. The inhibitory effect could be observed at a concentration of 100U/ml and was near to maximum at 1 000U/ml in acute phase, whereas the same suppressive action of IL 4 was reached at 100U/ml in recovery phase. When PBMCs were cultured in the presence of IL 4 at a concentration of 100U/ml, IL 4 down regulated the production of both TNF α and IL 1α mRNA by acute phase PBMCs only to 18.18～21.98% of control cells(in absence of IL 4), while the inhibitory rates were all near to 50% in recovery phases. Moreover, it was also found that the suppressive effect of IL 4 on the expression of PBMCs TNF α and IL 1α mRNA in the patients with endotoxinemia and HBeAg was significantly decreased in acute phase.Conclusions The inhibitory effect of IL 4 on the expression of PBMCs TNF α and IL 1α mRNA is markedly decreased in acute phase as compared with that in recovery phase and this declined response may be related to endotoxinemia and viremia.

关 键 词：病毒性肝炎 IL-4 TNF PBMCS 

分 类 号：R512.6[医药卫生—内科学]