脓毒症患者CD4^+CD25^+调节性T细胞检测的临床意义  

Clinic significance of Testing CD4^+CD25^+ Treg cells in patients with sepsis

作　　者：邵敏[1] 刘宝[1] 王锦权[1] 陶晓根[1] 周树生[1] 金魁[1] 张翠萍[2] 

机构地区：[1]安徽省立医院ICU,安徽合肥230001 [2]安徽省立医院中心实验室,安徽合肥230001

出　　处：《湖南中医药大学学报》2011年第4期8-10,共3页Journal of Hunan University of Chinese Medicine

摘　　要：目的探讨脓毒症患者CD4+CD25+调节性T细胞(Treg)的水平与机体细胞免疫状态的关系及其检测的临床意义。方法将脓毒症患者40例按照疾病严重程度分为3组:脓毒症组14例,严重脓毒症组15例,脓毒症休克组11例。所有入选的40例脓毒症患者在入选当天行流式细胞术检测血中CD4+CD25+调节性T细胞的比例和CD3+、CD4+、CD8+和CD4+/CD8+水平。比较脓毒症组、严重脓毒症组和脓毒症休克组血中CD4+CD25+调节性T细胞比例变化和CD3+、CD4+、CD8+和CD4+/CD8+水平变化。同时选取20例健康人为对照组。结果健康对照组CD4+CD25+调节性T细胞表达率脓毒症休克组最高,严重脓毒症组次之,脓毒症组最低,三组间两两比较差异有统计学意义(P<0.05)。脓毒症组CD3+、CD4+和CD4+/CD8+较正常对照组明显升高(P<0.05),CD8+较正常对照组差异不明显(P>0.05)。严重脓毒症组和脓毒症休克患者CD3+、CD4+、CD4+/CD8+较正常对照组明显降低(P<0.05),CD8+较正常对照组改变不明显(P>0.05)。结论脓毒症患者外周血CD4+CD25+调节性T细胞表达增加,可能导致CD3+、CD4+、CD4+/CD8+水平降低,免疫抑制加强。提示CD4+CD25+调节性T细胞在脓毒症的免疫发病机制中可能起着重要作用,对评估患者预后有临床价值。Objective To study the level and significance of CD4^＋CD25^＋ Treg cells and CD3^＋、CD4^＋、CD8^＋ cells in peripheral blood of patients with sepsis.Methods 40 patients with sepsis Patients were divided into three groups： sepsis group（n=14）,severe sepsis group（n=15）,and septic shock group（n=11）.The expression of CD4^＋CD25^＋ Treg cells and CD3^＋、CD4^＋ 、CD8^＋ cells of 40 patients on the day 1 was detected by flow cytometry.The comparision in the expression of CD4^＋CD25^＋ Treg cells and CD3^＋、CD4^＋、CD8^＋ cells within groups was made.Twenty healthy individuals served as controls.Results The expression rate.The expression of CD4^＋CD25^＋ Treg cells was the highest in septic shock group（P〈0.05） followed by the severe sepsis group（P〈0.05） and the sepsis group the lowest（P〈0.05）.The level of CD3^＋、CD4^＋ and CD4^＋/CD8^＋in sepsis group was higher than control group and the level of CD3^＋、CD4^＋ and CD4^＋/CD8^＋ in severe sepsis and sepsis shock was lower than control group.Conclusion The expression of CD4^＋CD25^＋ Treg cells was increased in sepsis patients and the level of CD3^＋、CD4^＋ and CD4^＋/CD8^＋was low in severe sepsis and sepsis shock patients,suggesting that they may play an important role in pathogenesis of sepsis.Blood Treg% level can reflect the cell immune state of patientswith sepsis and is of clinical value to assess the prognosis.

关 键 词：脓毒症 细胞免疫 CD4+CD25+调节性T细胞 

分 类 号：R631[医药卫生—外科学]